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Variations on a theme: crystal forms of the amino-acid transporter MhsT

DOI: 10.1107/S2053230X22007154 DOI Help

Authors: Caroline Neumann (Aarhus University; DANDRITE, Nordic EMBL Partnership for Molecular Medicine) , Dorota Focht (Aarhus University; DANDRITE, Nordic EMBL Partnership for Molecular Medicine) , Sofia Trampari (Aarhus University; DANDRITE, Nordic EMBL Partnership for Molecular Medicine) , Joseph A. Lyons (Aarhus University; DANDRITE, Nordic EMBL Partnership for Molecular Medicine) , Poul Nissen (Aarhus University; DANDRITE, Nordic EMBL Partnership for Molecular Medicine)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Acta Crystallographica Section F Structural Biology Communications , VOL 78

State: Published (Approved)
Published: August 2022

Open Access Open Access

Abstract: The bacterial amino-acid transporter MhsT from the SLC6A family has been crystallized in complex with different substrates in order to understand the determinants of the substrate specificity of the transporter. Surprisingly, crystals of the different MhsT–substrate complexes showed interrelated but different crystal-packing arrangements. Space-group assignment and structure determination of these different crystal forms present challenging combinations of pseudosymmetry, twinning and translational non­crystallographic symmetry.

Journal Keywords: pseudomerohedral twinning; pseudosymmetry; translational NCS; X-ray crystallography; translational symmetry; membrane proteins; bacterial amino-acid transporters; MhsT

Subject Areas: Biology and Bio-materials


Instruments: I04-Macromolecular Crystallography , I24-Microfocus Macromolecular Crystallography

Added On: 28/07/2022 14:47

Documents:
Acta Crystallographica F - 2022 - Neumann - Variations on a theme crystal forms of the amino%E2%80%90acid transporter MhsT.pdf

Discipline Tags:

Structural biology Life Sciences & Biotech

Technical Tags:

Diffraction Macromolecular Crystallography (MX)