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Using micro-synchrotron radiation x-ray fluorescence (µ-SRXRF) for trace metal imaging in the development of MRI contrast agents for prostate cancer imaging

DOI: 10.1016/j.jtemb.2022.127054 DOI Help

Authors: E. Dao (McMaster University; University of Tuebingen) , Maria V. Clavijo Jordan (University of Texas Southwestern Medical Center; Harvard Medical School) , K. Geraki (Diamond Light Source; University of Tuebingen) , A. F. Martins (University of Texas Southwestern Medical Center; University of Tuebingen) , S. Chirayil (University of Texas Southwestern Medical Center; University of Tuebingen) , A. D. Sherry (University of Texas Southwestern Medical Center; University of Tuebingen; University of Texas at Dallas) , M. J. Farquharson (University of Tuebingen; McMaster University)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Journal Of Trace Elements In Medicine And Biology , VOL 74

State: Published (Approved)
Published: December 2022
Diamond Proposal Number(s): 23705

Abstract: Background: Contrast agents (CA) are administered in magnetic resonance imaging (MRI) clinical exams to measure tissue perfusion, enhance image contrast between adjacent tissues, or provide additional biochemical information in molecular MRI. The efficacy of a CA is determined by the tissue distribution of the agent and its concentration in the extracellular space of all tissues. Methods: In this work, micro-synchrotron radiation x-ray fluorescence (µ-SRXRF) was used to examine and characterize a gadolinium-based zinc-sensitive agent (GdL2) currently under development for detection of prostate cancer (PCa) by MRI. Prostate tissue samples were collected from control mice and mice with known PCa after an MRI exam that included injection of GdL2. The samples were raster scanned to investigate trends in Zn, Gd, Cu, Fe, S, P, and Ca. Results: Significant Zn and Gd co-localization was observed in both healthy and malignant tissues. In addition, a marked decrease in Zn was found in the lateral lobe of the prostate obtained from mice with PCa. Conclusion: We demonstrate here that µ-SRXRF is a useful tool for monitoring the distribution of several elements including Zn and Gd in animal models of cancer. The optimized procedures for tissue preparation, processing, data collection, and analysis are described.

Journal Keywords: Contrast agent; Prostate cancer; Synchrotron radiation; X-ray fluorescence; Zinc; Gadolinium

Diamond Keywords: Prostate Cancer

Subject Areas: Biology and Bio-materials


Instruments: I18-Microfocus Spectroscopy

Added On: 10/08/2022 08:51

Discipline Tags:

Non-Communicable Diseases Health & Wellbeing Cancer Technique Development - Life Sciences & Biotech Life Sciences & Biotech

Technical Tags:

Imaging X-ray Fluorescence (XRF)