Periplasmic bacterial biomineralization of copper sulfide nanoparticles

DOI: 10.1002/advs.202203444

Authors: Yeseul Park (Aix-Marseille University, French Alternative Energies and Atomic Energy Commission (CEA), French National Center for Scientific Research (CNRS), UMR7265 Institute of Biosciences and Biotechnologies of Aix-Marseille (BIAM)) , Zohar Eyal (Weizmann Institute of Science) , Péter Pekker (University of Pannonia) , Daniel M. Chevrier (Aix-Marseille University, French Alternative Energies and Atomic Energy Commission (CEA), French National Center for Scientific Research (CNRS), UMR7265 Institute of Biosciences and Biotechnologies of Aix-Marseille (BIAM)) , Christopher T. Lefèvre (Aix-Marseille University, French Alternative Energies and Atomic Energy Commission (CEA), French National Center for Scientific Research (CNRS), UMR7265 Institute of Biosciences and Biotechnologies of Aix-Marseille (BIAM)) , Pascal Arnoux (Aix-Marseille University, French Alternative Energies and Atomic Energy Commission (CEA), French National Center for Scientific Research (CNRS), UMR7265 Institute of Biosciences and Biotechnologies of Aix-Marseille (BIAM)) , Jean Armengaud (Medicines and Healthcare Technologies Department (DMTS) University of Paris-Saclay, French Alternative Energies and Atomic Energy Commission (CEA), National Research Institute for Agriculture, Food and the Environment (INRAE), Pharmacology and Immunoanalysis unit (SPI)) , Caroline L. Monteil (Aix-Marseille University, French Alternative Energies and Atomic Energy Commission (CEA), French National Center for Scientific Research (CNRS), UMR7265 Institute of Biosciences and Biotechnologies of Aix-Marseille (BIAM)) , Assaf Gal (Weizmann Institute of Science) , Mihály Pósfai (University of Pannonia; ELKH-PE Environmental Mineralogy Research Group) , Damien Faivre (Aix-Marseille University, French Alternative Energies and Atomic Energy Commission (CEA), French National Center for Scientific Research (CNRS), UMR7265 Institute of Biosciences and Biotechnologies of Aix-Marseille (BIAM))
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Advanced Science , VOL 18

State: Published (Approved)
Published: August 2022
Diamond Proposal Number(s): 23693

Abstract: Metal sulfides are a common group of extracellular bacterial biominerals. However, only a few cases of intracellular biomineralization are reported in this group, mostly limited to greigite (Fe3S4) in magnetotactic bacteria. Here, a previously unknown periplasmic biomineralization of copper sulfide produced by the magnetotactic bacterium Desulfamplus magnetovallimortis strain BW-1, a species known to mineralize greigite (Fe3S4) and magnetite (Fe3O4) in the cytoplasm is reported. BW-1 produces hundreds of spherical nanoparticles, composed of 1–2 nm substructures of a poorly crystalline hexagonal copper sulfide structure that remains in a thermodynamically unstable state. The particles appear to be surrounded by an organic matrix as found from staining and electron microscopy inspection. Differential proteomics suggests that periplasmic proteins, such as a DegP-like protein and a heavy metal-binding protein, could be involved in this biomineralization process. The unexpected periplasmic formation of copper sulfide nanoparticles in BW-1 reveals previously unknown possibilities for intracellular biomineralization that involves intriguing biological control and holds promise for biological metal recovery in times of copper shortage.

Journal Keywords: biologically-controlled biomineralization; copper sulfide; cryo-electron tomography; intracellular biomineralization; magnetotactic bacteria; proteomics

Diamond Keywords: Biomineralisation; Bacteria; Bioleaching

Subject Areas: Chemistry, Biology and Bio-materials


Instruments: I14-Hard X-ray Nanoprobe

Other Facilities: MISTRAL at ALBA

Added On: 21/08/2022 16:18

Documents:
Advanced Science - 2022 - Park - Periplasmic Bacterial Biomineralization of Copper Sulfide Nanoparticles.pdf

Discipline Tags:

Biochemistry Chemistry Life Sciences & Biotech

Technical Tags:

Imaging Spectroscopy X-ray Fluorescence (XRF) X-ray Absorption Spectroscopy (XAS)