Three-dimensional electron ptychography of organic–inorganic hybrid nanostructures

DOI: 10.1038/s41467-022-32548-x

Authors: Zhiyuan Ding (Nanjing University; University of Oxford) , Si Gao (Nanjing University) , Weina Fang (East China Normal University) , Chen Huang (University of Oxford; The Rosalind Franklin Institute) , Liqi Zhou (Nanjing University) , Xudong Pei (Nanjing University) , Xiaoguo Liu (Shanghai Jiao Tong University) , Xiaoqing Pan (University of California, Irvine) , Chunhai Fan (Shanghai Jiao Tong University) , Angus I. Kirkland (University of Oxford; The Rosalind Franklin Institute; Diamond Light Source) , Peng Wang (Nanjing University; University of Warwick)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Nature Communications , VOL 13

State: Published (Approved)
Published: August 2022

Abstract: Three dimensional scaffolded DNA origami with inorganic nanoparticles has been used to create tailored multidimensional nanostructures. However, the image contrast of DNA is poorer than those of the heavy nanoparticles in conventional transmission electron microscopy at high defocus so that the biological and non-biological components in 3D scaffolds cannot be simultaneously resolved using tomography of samples in a native state. We demonstrate the use of electron ptychography to recover high contrast phase information from all components in a DNA origami scaffold without staining. We further quantitatively evaluate the enhancement of contrast in comparison with conventional transmission electron microscopy. In addition, We show that for ptychography post-reconstruction focusing simplifies the workflow and reduces electron dose and beam damage.

Journal Keywords: DNA nanotechnology; Organic–inorganic nanostructures; Transmission electron microscopy

Subject Areas: Technique Development, Materials, Biology and Bio-materials


Technical Areas:

Added On: 22/08/2022 10:34

Documents:
s41467-022-32548-x.pdf

Discipline Tags:

Biomaterials Technique Development - Materials Science Materials Science Nanoscience/Nanotechnology Life Sciences & Biotech

Technical Tags: