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A blast fungus zinc-finger fold effector binds to a hydrophobic pocket in host Exo70 proteins to modulate immune recognition in rice

DOI: 10.1073/pnas.2210559119 DOI Help

Authors: Juan Carlos De La Concepcion (John Innes Centre) , Koki Fujisaki (Iwate Biotechnology Research Center) , Adam R. Bentham (John Innes Centre) , Neftaly Cruz Mireles (John Innes Centre; University of East Anglia) , Victor Sanchez De Medina Hernandez (John Innes Centre) , Motoki Shimizu (Iwate Biotechnology Research Center) , David M. Lawson (John Innes Centre) , Sophien Kamoun (University of East Anglia) , Ryohei Terauchi (Iwate Biotechnology Research Center; Kyoto University) , Mark J. Banfield (John Innes Centre)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Proceedings Of The National Academy Of Sciences , VOL 119

State: Published (Approved)
Published: October 2022
Diamond Proposal Number(s): 18565

Open Access Open Access

Abstract: Exocytosis plays an important role in plant–microbe interactions, in both pathogenesis and symbiosis. Exo70 proteins are integral components of the exocyst, an octameric complex that mediates tethering of vesicles to membranes in eukaryotes. Although plant Exo70s are known to be targeted by pathogen effectors, the underpinning molecular mechanisms and the impact of this interaction on infection are poorly understood. Here, we show the molecular basis of the association between the effector AVR-Pii of the blast fungus Maganaporthe oryzae and rice Exo70 alleles OsExo70F2 and OsExo70F3, which is sensed by the immune receptor pair Pii via an integrated RIN4/NOI domain. The crystal structure of AVR-Pii in complex with OsExo70F2 reveals that the effector binds to a conserved hydrophobic pocket in Exo70, defining an effector/target binding interface. Structure-guided and random mutagenesis validates the importance of AVR-Pii residues at the Exo70 binding interface to sustain protein association and disease resistance in rice when challenged with fungal strains expressing effector mutants. Furthermore, the structure of AVR-Pii defines a zinc-finger effector fold (ZiF) distinct from the MAX (Magnaporthe Avrs and ToxB-like) fold previously described for a majority of characterized M. oryzae effectors. Our data suggest that blast fungus ZiF effectors bind a conserved Exo70 interface to manipulate plant exocytosis and that these effectors are also baited by plant immune receptors, pointing to new opportunities for engineering disease resistance.

Journal Keywords: effector; plant immunity; NLR; Exocyst

Diamond Keywords: Cereal Crops

Subject Areas: Biology and Bio-materials

Instruments: I03-Macromolecular Crystallography

Added On: 24/10/2022 09:18


Discipline Tags:

Plant science Pathogens Agriculture & Fisheries Structural biology Life Sciences & Biotech

Technical Tags:

Diffraction Macromolecular Crystallography (MX)