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Multilevel X-ray imaging approach to assess the sequential evolution of multi-organ damage in multiple sclerosis

DOI: 10.1038/s42005-022-01070-3 DOI Help

Authors: Francesca Palermo (Institute of Nanotechnology-CNR) , Nicola Pieroni (Institute of Nanotechnology-CNR) , Alessia Sanna (Institute of Nanotechnology-CNR; Université Grenoble Alpes) , Benedetta Parodi (University of Genoa) , Consuelo Venturi (University of Genoa) , Ginevra Begani Provinciali (Institute of Nanotechnology-CNR; CNRS, Ecole Polytechnique IP Paris) , Lorenzo Massimi (Institute of Nanotechnology-CNR) , Laura Maugeri (Institute of Nanotechnology-CNR) , Gian Paolo Marra (Institute of Nanotechnology-CNR) , Elena Longo (Elettra - Sincrotrone Trieste) , Lorenzo D’amico (Elettra - Sincrotrone Trieste; University of Trieste) , Giulia Saccomano (Elettra - Sincrotrone Trieste; University of Trieste) , Jonathan Perrin (Synchrotron SOLEIL) , Giuliana Tromba (Elettra - Sincrotrone Trieste) , Inna Bukreeva (Institute of Nanotechnology-CNR) , Michela Fratini (Institute of Nanotechnology-CNR; IRCCS Fondazione Santa Lucia) , Giuseppe Gigli (Institute of Nanotechnology-CNR) , Nicole Kerlero De Rosbo (Institute of Nanotechnology-CNR; University of Genoa) , Alessia Cedola (Institute of Nanotechnology-CNR)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Communications Physics , VOL 5

State: Published (Approved)
Published: November 2022
Diamond Proposal Number(s): 23997

Open Access Open Access

Abstract: The 3D complexity of biological tissues and intricate structural-functional connections call for state-of-the-art X-ray imaging approaches to overcome limitations of classical imaging. Unlike other imaging techniques, X-ray phase-contrast tomography (XPCT) offers a highly sensitive 3D imaging approach to investigate different disease-relevant networks at levels ranging from single cell through to intact organ. We present here a concomitant study of the evolution of tissue damage and inflammation in different organs affected by the disease in the murine model for multiple sclerosis, a demyelinating autoimmune disorder of the central nervous system. XPCT identifies and monitors structural and cellular alterations throughout the central nervous system, but also in the gut, and eye, of mice induced to develop multiple sclerosis-like disease and sacrificed at pre-symptomatic and symptomatic time points. This study details the sequential evolution of multi-organ damages in the murine multiple sclerosis model showing the disease development and progression which is of relevance for the human case.

Diamond Keywords: Multiple Sclerosis

Subject Areas: Biology and Bio-materials, Technique Development

Instruments: I13-2-Diamond Manchester Imaging

Other Facilities: ID17 at ESRF; ANATOMIX at SOLEIL; SYRMEP at Elettra

Added On: 22/11/2022 10:13

Discipline Tags:

Neurodegenerative Diseases Non-Communicable Diseases Health & Wellbeing Technique Development - Life Sciences & Biotech Neurology Biophysics Life Sciences & Biotech

Technical Tags:

Imaging Tomography