Mechanisms of E. coli chemotaxis signaling pathways visualized using cryoET and computational approaches

DOI: 10.1042/BST20220191

Authors: Kyprianos Hadjidemetriou (Diamond Light Source) , Satinder Kaur (Diamond Light Source) , C. Keith Cassidy (Diamond Light Source) , Peijun Zhang (Diamond Light Source; Wellcome Trust Centre for Human Genetics, University of Oxford; Chinese Academy of Medical Sciences Oxford Institute, University of Oxford)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Biochemical Society Transactions , VOL 153

State: Published (Approved)
Published: November 2022

Abstract: Chemotaxis signaling pathways enable bacteria to sense and respond to their chemical environment and, in some species, are critical for lifestyle processes such as biofilm formation and pathogenesis. The signal transduction underlying chemotaxis behavior is mediated by large, highly ordered protein complexes known as chemosensory arrays. For nearly two decades, cryo-electron tomography (cryoET) has been used to image chemosensory arrays, providing an increasingly detailed understanding of their structure and function. In this mini-review, we provide an overview of the use of cryoET to study chemosensory arrays, including imaging strategies, key results, and outstanding questions. We further discuss the application of molecular modeling and simulation techniques to complement structure determination efforts and provide insight into signaling mechanisms. We close the review with a brief outlook, highlighting promising future directions for the field.

Journal Keywords: chemoreceptor; chemosensory array; chemotaxis; core signaling complex; cryo-electron tomography; cryoEM

Diamond Keywords: Bacteria

Subject Areas: Biology and Bio-materials


Technical Areas:

Added On: 28/11/2022 11:19

Discipline Tags:

Structural biology Life Sciences & Biotech

Technical Tags: