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Ex vivo evaluation of ethosomes and transethosomes applied on human skin: a comparative study

DOI: 10.3390/ijms232315112 DOI Help

Authors: Elisabetta Esposito (University of Ferrara) , Laura Calderan (University of Verona) , Andrea Galvan (University of Verona) , Enrica Cappellozza (University of Verona) , Markus Drechsler (University of Bayreuth) , Paolo Mariani (Università Politecnica delle Marche) , Alessia Pepe (Università Politecnica delle Marche) , Maddalena Sguizzato (University of Ferrara) , Enrico Vigato (Verona University Hospital (A.O.U.I. Verona)) , Edoardo Dalla Pozza (Verona University Hospital (A.O.U.I. Verona)) , Manuela Malatesta (University of Verona)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: International Journal Of Molecular Sciences , VOL 23

State: Published (Approved)
Published: December 2022
Diamond Proposal Number(s): 21035

Open Access Open Access

Abstract: In this study, the transdermal fate of vesicular nanosystems was investigated. Particularly, ethosomes based on phosphatidylcholine 0.9% w/w and transethosomes based on phosphatidylcholine 0.9 or 2.7% w/w plus polysorbate 80 0.3% w/w as an edge activator were prepared and characterized. The vesicle mean size, morphology and deformability were influenced by both phosphatidylcholine and polysorbate 80. Indeed, the mean diameters of ethosome were around 200 nm, while transethosome’s mean diameters were 146 or 350 nm in the case of phosphatidylcholine 0.9 or 2.7%, w/w, respectively. The highest deformability was achieved by transethosomes based on phosphatidylcholine 0.9%, w/w. The three types of vesicular nanosystems were applied on explanted human skin maintained in a bioreactor. Transmission electron microscopy demonstrated that all vesicles were able to enter the skin, keeping their structural integrity. Notably, the vesicle penetration capability was influenced by their physical-chemical features. Indeed, ethosomes reached keratinocytes and even the dermis, phosphatidylcholine 0.9% transethosomes were found in keratinocytes and phosphatidylcholine 2.7% transethosomes were found only in corneocytes of the outer layer. These findings open interesting perspectives for a differentiated application of these vesicles for transdermal drug delivery as a function of the cutaneous pathology to be addressed.

Journal Keywords: ethosomes; transethosomes; explanted skin; bioreactor; X-ray diffraction; cryogenic transmission electron microscopy; transmission electron microscopy

Subject Areas: Medicine, Biology and Bio-materials


Instruments: B21-High Throughput SAXS

Added On: 08/12/2022 10:18

Documents:
ijms-23-15112.pdf

Discipline Tags:

Drug Delivery Health & Wellbeing Life Sciences & Biotech

Technical Tags:

Scattering Small Angle X-ray Scattering (SAXS)