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Metabolic reprogramming in colon cancer cells persistently infected with Newcastle disease virus

DOI: 10.3390/cancers15030811 DOI Help

Authors: Tong Yu (University of Oxford) , Archana Chandrabhan Jadhav (Diamond Light Source; The Pirbright Institute) , Jiabao Xu (University of Oxford) , Adrian L. Harris (John Radcliffe Hospital, Oxford University) , Venugopal Nair (The Pirbright Institute; University of Oxford) , Wei E. Huang (University of Oxford)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Cancers , VOL 15

State: Published (Approved)
Published: January 2023

Open Access Open Access

Abstract: Newcastle disease virus (NDV) is an oncolytic agent against various types of mammalian cancers. As with all cancer therapies, the development of cancer resistance, both innate and acquired, is becoming a challenge. In this study, we investigated persistently NDV-infected Caco-2 colon cancer cells, designated as virus-resistant (VR) Caco-2 cells, which were then able to resist NDV-mediated oncolysis. We applied single-cell Raman spectroscopy, combined with deuterium isotope probing (Raman-DIP) techniques, to investigate the metabolic adaptations and dynamics in VR Caco-2 cells. A linear discriminant analysis (LDA) model demonstrated excellent performance in differentiating VR Caco-2 from Caco-2 cells at single-cell level. By comparing the metabolic profiles in a time-resolved manner, the de novo synthesis of proteins and lipids was found upregulated, along with decreased DNA synthesis in VR Caco-2. The results suggest that VR Caco-2 cells might reprogram their metabolism and divert energy from proliferation to protein synthesis and lipidic modulation. The ability to identify and characterise single resistant cells among a population of cancer cells would help develop a deeper understanding of the resistance mechanisms and better tactics for developing effective cancer treatment.

Journal Keywords: Newcastle disease virus; Raman spectroscopy; cancer resistance; drug resistance; single cells

Diamond Keywords: Newcastle Disease Virus; Viruses; Colon Cancer

Subject Areas: Biology and Bio-materials, Medicine


Technical Areas:

Added On: 31/01/2023 10:14

Documents:
cancers-15-00811-v2.pdf

Discipline Tags:

Non-Communicable Diseases Health & Wellbeing Cancer Drug Discovery Life Sciences & Biotech

Technical Tags: