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Structure and function of microbial α- l -fucosidases: a mini review

DOI: 10.1042/EBC20220158 DOI Help

Authors: Haiyang Wu (Institute of Biological and Medical Engineering, Guangdong Academy of Sciences) , C. David Owen (Diamond Light Source; Research Complex at Harwell) , Nathalie Juge (Quadram Institute Bioscience)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Essays In Biochemistry , VOL 39

State: Published (Approved)
Published: February 2023

Open Access Open Access

Abstract: Fucose is a monosaccharide commonly found in mammalian, insect, microbial and plant glycans. The removal of terminal α-l-fucosyl residues from oligosaccharides and glycoconjugates is catalysed by α-l-fucosidases. To date, glycoside hydrolases (GHs) with exo-fucosidase activity on α-l-fucosylated substrates (EC 3.2.1.51, EC 3.2.1.-) have been reported in the GH29, GH95, GH139, GH141 and GH151 families of the Carbohydrate Active Enzymes (CAZy) database. Microbes generally encode several fucosidases in their genomes, often from more than one GH family, reflecting the high diversity of naturally occuring fucosylated structures they encounter. Functionally characterised microbial α-l-fucosidases have been shown to act on a range of substrates with α-1,2, α-1,3, α-1,4 or α-1,6 fucosylated linkages depending on the GH family and microorganism. Fucosidases show a modular organisation with catalytic domains of GH29 and GH151 displaying a (β/α)8-barrel fold while GH95 and GH141 show a (α/α)6 barrel and parallel β-helix fold, respectively. A number of crystal structures have been solved in complex with ligands, providing structural basis for their substrate specificity. Fucosidases can also be used in transglycosylation reactions to synthesise oligosaccharides. This mini review provides an overview of the enzymatic and structural properties of microbial α-l-fucosidases and some insights into their biological function and biotechnological applications.

Journal Keywords: carbohydrate-active enzymes; fucose; fucosidases; glycoside hydrolases; gut bacteria

Diamond Keywords: Bacteria; Gut Microbiota; Enzymes

Subject Areas: Biology and Bio-materials


Technical Areas:

Added On: 27/02/2023 09:11

Documents:
ebc-2022-0158c.pdf

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Structural biology Life Sciences & Biotech

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