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Revealing low-dose radiation damage using single-crystal spectroscopy

DOI: 10.1107/S0909049511004250 DOI Help
PMID: 21525644 PMID Help

Authors: Robin L. Owen (Diamond Light Source) , Briony A. Yorke (University of Leeds) , James A. Gowdy (University of Leeds) , Arwen R. Pearson (University of Leeds)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Journal Of Synchrotron Radiation , VOL 18 (3) , PAGES 367-373

State: Published (Approved)
Published: May 2011

Open Access Open Access

Abstract: The structural information and functional insight obtained from X-ray crystallography can be enhanced by the use of complementary spectroscopies. Here the information that can be obtained from spectroscopic methods commonly used in conjunction with X-ray crystallography and best-practice single-crystal UV-Vis absorption data collection are briefly reviewed. Using data collected with the in situ system at the Swiss Light Source, the time and dose scales of low-dose X-ray-induced radiation damage and solvated electron generation in metalloproteins at 100 K are investigated. The effect of dose rate on these scales is also discussed.

Journal Keywords: Crystallography; Damage; Monocrystals; Myoglobin; Radiation Effects; Spectroscopy; X Radiation

Subject Areas: Technique Development

Facility: SLS

Added On: 30/03/2011 09:29

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