Sample preparation of atherosclerotic plaque for SAXS/WAXS experimentation

DOI: 10.1021/acsomega.3c00060

Authors: Rebecca R. Mackley (University of Warwick) , Steven Huband (University of Warwick) , Tara L. Schiller (University of Warwick)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Acs Omega

State: Published (Approved)
Published: April 2023
Diamond Proposal Number(s): 27756 , 29720

Abstract: Atherosclerosis is often described as a single disease entity; however, the morphology of each plaque is unique to the individual. The field currently lacks a technique that can discriminate stable from unstable plaques, to identify those at risk of a thromboembolic event. Small- and wide-angle X-ray scattering (SAXS/WAXS) holds the potential to be able to identify key materials present in a plaque, such as cholesterol species, collagen, low-density lipoproteins (LDLs), and hydroxyapatite. Protocols have been established for the preparation of excised human atherosclerotic tissue that are investigated herein. This includes the fixing, sectioning, and substrate selection of the sample. Through several sample preparation methods, vast improvements have been made to sample-to-noise ratio and background subtraction.

Diamond Keywords: Atherosclerosis; Cardiovascular Disease

Subject Areas: Technique Development, Biology and Bio-materials


Instruments: B21-High Throughput SAXS , DL-SAXS-Offline SAXS and Sample Environment Development

Other Facilities: SAXS/WAXS beamline at Australian Synchrotron

Added On: 06/04/2023 14:34

Documents:
acsomega.3c00060.pdf

Discipline Tags:

Non-Communicable Diseases Health & Wellbeing Technique Development - Life Sciences & Biotech Life Sciences & Biotech

Technical Tags:

Scattering Small Angle X-ray Scattering (SAXS) Wide Angle X-ray Scattering (WAXS)