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Structural analysis of Bacillus subtilis sigma factors

DOI: 10.3390/microorganisms11041077 DOI Help

Authors: Katherine M. Collins (King's College London) , Nicola J. Evans (King’s College London) , James H. Torpey (King's College London) , Jonathon M. Harris (King’s College London) , Bethany A. Haynes (King’s College London) , Amy H. Camp (Mount Holyoke College) , Rivka L. Isaacson (King's College London)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Microorganisms , VOL 11

State: Published (Approved)
Published: April 2023
Diamond Proposal Number(s): 13597

Open Access Open Access

Abstract: Bacteria use an array of sigma factors to regulate gene expression during different stages of their life cycles. Full-length, atomic-level structures of sigma factors have been challenging to obtain experimentally as a result of their many regions of intrinsic disorder. AlphaFold has now supplied plausible full-length models for most sigma factors. Here we discuss the current understanding of the structures and functions of sigma factors in the model organism, Bacillus subtilis, and present an X-ray crystal structure of a region of B. subtilis SigE, a sigma factor that plays a critical role in the developmental process of spore formation.

Journal Keywords: Bacillus subtilis; sigma factor; SigE; X-ray crystallography; structure; Alphafold; sporulation

Diamond Keywords: Bacteria

Subject Areas: Biology and Bio-materials

Instruments: I03-Macromolecular Crystallography

Added On: 26/04/2023 08:45


Discipline Tags:

Genetics Structural biology Life Sciences & Biotech

Technical Tags:

Diffraction Macromolecular Crystallography (MX)