Histidine-containing amphiphilic peptide-based non-cytotoxic hydrogelator with antibacterial activity and sustainable drug release

DOI: 10.1021/acs.langmuir.3c00235

Authors: Biswanath Hansda (Indian Association for the Cultivation of Science) , Jhilam Majumder (Jadavpur University) , Biplab Mondal (Indian Association for the Cultivation of Science) , Akash Chatterjee (Indian Association for the Cultivation of Science) , Subhadeep Das (Purdue University) , Sourav Kumar (Bose Institute) , Ratan Gachhui (Jadavpur University) , Valeria Castelletto (University of Reading) , Ian W. Hamley (University of Reading) , Prosenjit Sen (Indian Association for the Cultivation of Science) , Arindam Banerjee (Indian Association for the Cultivation of Science)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Langmuir

State: Published (Approved)
Published: May 2023
Diamond Proposal Number(s): 29895

Abstract: A histidine-based amphiphilic peptide (P) has been found to form an injectable transparent hydrogel in phosphate buffer solution over a pH range from 7.0 to 8.5 with an inherent antibacterial property. It also formed a hydrogel in water at pH = 6.7. The peptide self-assembles into a nanofibrillar network structure which is characterized by high-resolution transmission electron microscopy, field-emission scanning electron microscopy, atomic force microscopy, small-angle X-ray scattering, Fourier-transform infrared spectroscopy, and wide-angle powder X-ray diffraction. The hydrogel exhibits efficient antibacterial activity against both Gram-positive bacteria Staphylococcus aureus (S. aureus) and Gram-negative bacteria Escherichia coli (E. coli). The minimum inhibitory concentration of the hydrogel ranges from 20 to 100 μg/mL. The hydrogel is capable of encapsulation of the drugs naproxen (a non-steroidal anti-inflammatory drug), amoxicillin (an antibiotic), and doxorubicin, (an anticancer drug), but, selectively and sustainably, the gel releases naproxen, 84% being released in 84 h and amoxicillin was released more or less in same manner with that of the naproxen. The hydrogel is biocompatible with HEK 293T cells as well as NIH (mouse fibroblast cell line) cells and thus has potential as a potent antibacterial and drug releasing agent. Another remarkable feature of this hydrogel is its magnification property like a convex lens.

Diamond Keywords: Bacteria

Subject Areas: Biology and Bio-materials, Materials, Medicine


Instruments: B21-High Throughput SAXS

Added On: 22/05/2023 10:12

Discipline Tags:

Drug Delivery Antibiotic Resistance Health & Wellbeing Materials Science Polymer Science Life Sciences & Biotech

Technical Tags:

Scattering Small Angle X-ray Scattering (SAXS)