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Effector target-guided engineering of an integrated domain expands the disease resistance profile of a rice NLR immune receptor

DOI: 10.7554/eLife.81123 DOI Help

Authors: Josephine H. R. Maidment (John Innes Centre) , Motoki Shimizu (Iwate Biotechnology Research Center) , Adam R. Bentham (John Innes Centre) , Sham Vera (John Innes Centre) , Marina Franceschetti (John Innes Centre) , Apinya Longya (John Innes Centre) , Clare E. M. Stevenson (John Innes Centre) , Juan Carlos De La Concepcion (John Innes Centre) , Aleksandra Bialas (University of East Anglia) , Sophien Kamoun (University of East Anglia) , Ryohei Terauchi (Iwate Biotechnology Research Center; Kyoto University) , Mark J. Banfield (John Innes Centre)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Elife , VOL 12

State: Published (Approved)
Published: May 2023
Diamond Proposal Number(s): 13467 , 18565

Open Access Open Access

Abstract: A subset of plant intracellular NLR immune receptors detect effector proteins, secreted by phytopathogens to promote infection, through unconventional integrated domains which resemble the effector’s host targets. Direct binding of effectors to these integrated domains activates plant defenses. The rice NLR receptor Pik-1 binds the Magnaporthe oryzae effector AVR-Pik through an integrated heavy metal-associated (HMA) domain. However, the stealthy alleles AVR-PikC and AVR-PikF avoid interaction with Pik-HMA and evade host defenses. Here, we exploited knowledge of the biochemical interactions between AVR-Pik and its host target, OsHIPP19, to engineer novel Pik-1 variants that respond to AVR-PikC/F. First, we exchanged the HMA domain of Pikp-1 for OsHIPP19-HMA, demonstrating that effector targets can be incorporated into NLR receptors to provide novel recognition profiles. Second, we used the structure of OsHIPP19-HMA to guide the mutagenesis of Pikp-HMA to expand its recognition profile. We demonstrate that the extended recognition profiles of engineered Pikp-1 variants correlate with effector binding in planta and in vitro, and with the gain of new contacts across the effector/HMA interface. Crucially, transgenic rice producing the engineered Pikp-1 variants was resistant to blast fungus isolates carrying AVR-PikC or AVR-PikF. These results demonstrate that effector target-guided engineering of NLR receptors can provide new-to-nature disease resistance in crops.

Diamond Keywords: Cereal Crops; Fungi

Subject Areas: Biology and Bio-materials

Instruments: I03-Macromolecular Crystallography , I04-Macromolecular Crystallography

Added On: 24/05/2023 11:00

Discipline Tags:

Plant science Pathogens Agriculture & Fisheries Structural biology Life Sciences & Biotech

Technical Tags:

Diffraction Macromolecular Crystallography (MX)