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Cryogenic electron ptychographic single particle analysis with wide bandwidth information transfer

DOI: 10.1038/s41467-023-38268-0 DOI Help

Authors: Xudong Pei (Nanjing University) , Liqi Zhou (Nanjing University; University of Warwick) , Chen Huang (The Rosalind Franklin Institute) , Mark Boyce (Wellcome Trust Centre for Human Genetics, University of Oxford) , Judy S. Kim (The Rosalind Franklin Institute; University of Oxford) , Emanuela Liberti (The Rosalind Franklin Institute) , Yiming Hu (Nanjing University) , Takeo Sasaki (JEOL Ltd) , Peter D. Nellist (University of Oxford) , Peijun Zhang (Welcome Trust Centre for Human Genetics, University of Oxford; Diamond Light Source) , David I. Stuart (Welcome Trust Centre for Human Genetics, University of Oxford; Diamond Light Source) , Angus I. Kirkland (The Rosalind Franklin Institute; University of Oxford; Diamond Light Source) , Peng Wang (Nanjing University; University of Warwick)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Nature Communications , VOL 14

State: Published (Approved)
Published: May 2023
Diamond Proposal Number(s): 17918

Open Access Open Access

Abstract: Advances in cryogenic transmission electron microscopy have revolutionised the determination of many macromolecular structures at atomic or near-atomic resolution. This method is based on conventional defocused phase contrast imaging. However, it has limitations of weaker contrast for small biological molecules embedded in vitreous ice, in comparison with cryo-ptychography, which shows increased contrast. Here we report a single-particle analysis based on the use of ptychographic reconstruction data, demonstrating that three dimensional reconstructions with a wide information transfer bandwidth can be recovered by Fourier domain synthesis. Our work suggests future applications in otherwise challenging single particle analyses, including small macromolecules and heterogeneous or flexible particles. In addition structure determination in situ within cells without the requirement for protein purification and expression may be possible.

Subject Areas: Technique Development, Biology and Bio-materials

Diamond Offline Facilities: Electron Physical Sciences Imaging Centre (ePSIC)
Instruments: E02-JEM ARM 300CF

Added On: 31/05/2023 08:49


Discipline Tags:

Technique Development - Life Sciences & Biotech Life Sciences & Biotech

Technical Tags:

Imaging Coherent Diffraction Imaging (CDI) Ptychography