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A monomeric StayGold fluorescent protein

DOI: 10.1038/s41587-023-02018-w DOI Help

Authors: Esther Ivorra-Molla (University of Warwick) , Dipayan Akhuli (University of Warwick; Indian Institute of Science) , Martin B. L. Mcandrew (University of Warwick) , William Scott (University of Warwick) , Lokesh Kumar (University of Warwick) , Saravanan Palani (Indian Institute of Science) , Masanori Mishima (University of Warwick) , Allister Crow (University of Warwick) , Mohan K. Balasubramanian (University of Warwick)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Nature Biotechnology , VOL 40

State: Published (Approved)
Published: December 2023

Open Access Open Access

Abstract: StayGold is an exceptionally bright and stable fluorescent protein that is highly resistant to photobleaching. Despite favorable fluorescence properties, use of StayGold as a fluorescent tag is limited because it forms a natural dimer. Here we report the 1.6 Å structure of StayGold and generate a derivative, mStayGold, that retains the brightness and photostability of the original protein while being fully monomeric.

Subject Areas: Biology and Bio-materials

Instruments: I04-Macromolecular Crystallography

Added On: 13/12/2023 14:00


Discipline Tags:

Structural biology Life Sciences & Biotech

Technical Tags:

Diffraction Macromolecular Crystallography (MX)