Hyperspectral mapping of human primary and stem cells at cell-matrix interfaces

DOI: 10.1021/acsami.3c17113

Authors: Emiliana De Santis (National Physical Laboratory (UK)) , Nilofar Faruqui (National Physical Laboratory (UK)) , Craig T. Russell (National Physical Laboratory (UK)) , James E. Noble (National Physical Laboratory (UK)) , Ibolya E. Kepiro (National Physical Laboratory (UK)) , Katharine Hammond (National Physical Laboratory (UK)) , Maria Tsalenchuk (Imperial College London) , Eugeni M. Ryadnov (University College London) , Magda Wolna (Diamond Light Source) , Mark D. Frogley (Diamond Light Source) , Christopher J. Price (University of Sheffield) , Ivana Barbaric (University of Sheffield) , Gianfelice Cinque (Diamond Light Source) , Maxim G. Ryadnov (National Physical Laboratory (UK); King's College London)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Acs Applied Materials & Interfaces

State: Published (Approved)
Published: January 2024
Diamond Proposal Number(s): 16548

Abstract: Extracellular matrices interface with cells to promote cell growth and tissue development. Given this critical role, matrix mimetics are introduced to enable biomedical materials ranging from tissue engineering scaffolds and tumor models to organoids for drug screening and implant surface coatings. Traditional microscopy methods are used to evaluate such materials in their ability to support exploitable cell responses, which are expressed in changes in cell proliferation rates and morphology. However, the physical imaging methods do not capture the chemistry of cells at cell–matrix interfaces. Herein, we report hyperspectral imaging to map the chemistry of human primary and embryonic stem cells grown on matrix materials, both native and artificial. We provide the statistical analysis of changes in lipid and protein content of the cells obtained from infrared spectral maps to conclude matrix morphologies as a major determinant of biochemical cell responses. The study demonstrates an effective methodology for evaluating bespoke matrix materials directly at cell–matrix interfaces.

Diamond Keywords: Regenerative Medicine

Subject Areas: Biology and Bio-materials, Chemistry


Instruments: B22-Multimode InfraRed imaging And Microspectroscopy

Added On: 08/01/2024 09:21

Discipline Tags:

Biochemistry Chemistry Biophysics Life Sciences & Biotech

Technical Tags:

Spectroscopy Infrared Spectroscopy Synchtron-based Fourier Transform Infrared Spectroscopy (SR-FTIR)