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Biochemical and structural studies of LjSK1, a Lotus japonicusGSK3β/SHAGGY-like kinase, reveal its functional role

DOI: 10.1021/acs.jafc.3c07101 DOI Help

Authors: Theodora G. A. Solovou (University of Thessaly) , Georgios Stravodimos (University of Thessaly) , Georgios E. Papadopoulos (University of Thessaly) , Vassiliki T. Skamnaki (University of Thessaly) , Kalliope Papadopoulou (University of Thessaly) , Demetres D. Leonidas (University of Thessaly)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Journal Of Agricultural And Food Chemistry

State: Published (Approved)
Published: February 2024

Abstract: The crystal structure of a truncated form of the Lotus japonicus glycogen synthase kinase 3β (GSK3β) like kinase (LjSK190–467) has been resolved at 2.9 Å resolution, providing, for the first time, structural data for a plant GKS3β like kinase. The 3D structure of LjSK190–467 revealed conservation at the structural level for this plant member of the GSK3β family. However, comparative structural analysis to the human homologue revealed significant differences at the N- and C-termini, supporting the notion for an additional regulatory mechanism in plant GSK3-like kinases. Structural similarities at the catalytic site and the ATP binding site explained the similarity in the function of the human and plant protein. LjSK1 and lupeol are strongly linked to symbiotic bacterial infection and nodulation initiation. An inhibitory capacity of lupeol (IC50 = 0.77 μM) for LjSK1 was discovered, providing a biochemical explanation for the involvement of these two molecules in nodule formation, and constituted LjSK1 as a molecular target for the discovery of small molecule modulators for crop protection and development. Studies on the inhibitory capacity of two phytogenic triterpenoids (betulinic acid and hederacoside C) to LjSK1 provided their structure–activity relationship and showed that hederacoside C can be the starting point for such endeavors.

Subject Areas: Biology and Bio-materials, Chemistry


Instruments: I03-Macromolecular Crystallography

Added On: 12/02/2024 08:25

Discipline Tags:

Plant science Biochemistry Agriculture & Fisheries Chemistry Structural biology Life Sciences & Biotech

Technical Tags:

Diffraction Macromolecular Crystallography (MX)