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From SPINE to SPINE-2 complexes and beyond

DOI: 10.1016/j.jsb.2011.05.013 DOI Help

Authors: Anastassis Perrakis (Netherlands Cancer Institute) , Susan Daenke (University of Oxford) , David I. Stuart (Diamond Light Source) , Joel L. Sussman (The Israel Structural Proteomics Center, Weizmann Institute of Science)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Journal Of Structural Biology , VOL 175 (2)

State: Published (Approved)
Published: May 2011

Open Access Open Access

Abstract: It is about a decade since SPINE – Structural Proteomics IN Europe – was envisaged, set-up and submitted as an idea to the European Commission fifth framework programme. Our concepts were built on the paradigm of the USA-based structural genomics projects (http://www.nigms.nih.gov/psi), the Japanese initiatives (http://www.rsgi.riken.go.jp/rsgi_e), and some national efforts within Europe. At the same time, benefiting from the experience and technology development of the preceding projects, SPINE took an approach different from that of the mainstream of structural genomics: a major success of SPINE was to bring the cutting-edge high-throughput (HTP) technologies to biomedically relevant targets, not only facilitating the determination of no less than 308 novel structures, but also achieving a highly collaborative spirit and a culture of exchange of knowledge in technology. SPINE-2 Complexes (S2C) was both a continuation of SPINE and a whole new venture in its own right. Having largely achieved the integration of HTP technologies at partner sites, S2C aimed to facilitate the study of macromolecular complexes in key areas of biological research (ubiquitin (de-)conjugation, cell cycle and apoptosis, synaptogenesis and neuronal signaling, kinases and phosphatases, transcription receptors and regulation, innate and acquired immunity, and mechanisms in viral infection). S2C also worked to advance ‘wet-lab’ technologies relevant to the study of challenging targets (e.g., production of multi-subunit complexes with an emphasis on eukaryotic systems, biophysical methods to characterize complexes, and library tools for HTP screening), and ‘dry-lab’ technologies (e.g., microcrystal handling, docking software, as well as data management, analysis and dissemination tools). This ambitious agenda was backed-up by the long standing interests of the participant labs in the corresponding research themes, and was supported by a shared enthusiasm for consolidation of technologies and conceptual approaches, which together allowed us to showcase the structural study of complex systems.

Subject Areas: Biology and Bio-materials


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Added On: 05/07/2011 11:30

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