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Structural features underlying the selectivity of the kinase inhibitors NBC and dNBC: role of a nitro group that discriminates between CK2 and DYRK1A

DOI: 10.1007/s00018-011-0758-7 DOI Help

Authors: Stefania Sarno (Unknown) , Marco Mazzorana (Diamond Light Source) , Ryan Traynor (Medical Research Council Protein Phosphorylation Unit, University of Dundee,) , Maria Ruzzene (University of Padua; Venetian Institute of Molecular Medicine (VIMM)) , Giorgio Cozza (University of Padua) , Mario A. Pagano (University of Padua) , Flavio Meggio (University of Padua) , Giuseppe Zagotto (University of Padua) , Roberto Battistutta (Venetian Institute of Molecular Medicine (VIMM)) , Lorenzo A. Pinna (University of Padua)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Cellular And Molecular Life Sciences

State: Published (Approved)
Published: July 2011

Abstract: 8-hydroxy-4-methyl-9-nitrobenzo(g)chromen-2-one (NBC) has been found to be a fairly potent ATP site-directed inhibitor of protein kinase CK2 (Ki = 0.22 μM). Here, we show that NBC also inhibits PIM kinases, especially PIM1 and PIM3, the latter as potently as CK2. Upon removal of the nitro group, to give 8-hydroxy-4-methyl-benzo(g)chromen-2-one (here referred to as “denitro NBC”, dNBC), the inhibitory power toward CK2 is almost entirely lost (IC50 > 30 μM) whereas that toward PIM1 and PIM3 is maintained; in addition, dNBC is a potent inhibitor of a number of other kinases that are weakly inhibited or unaffected by NBC, with special reference to DYRK1A whose IC50 values with NBC and dNBC are 15 and 0.60 μM, respectively. Therefore, the observation that NBC, unlike dNBC, is a potent inducer of apoptosis is consistent with the notion that this effect is mediated by inhibition of endogenous CK2. The structural features underlying NBC selectivity have been revealed by inspecting its 3D structure in complex with the catalytic subunit of Z. mays CK2. The crucial role of the nitro group is exerted both through a direct electrostatic interaction with the side chain of Lys68 and, indirectly, by enhancing the acidic dissociation constant of the adjacent hydroxyl group which interacts with a conserved water molecule in the deepest part of the cavity. By contrast, the very same nitro group is deleterious for the binding to the active site of DYRK1A, as disclosed by molecular docking. This provides the rationale for preferential inhibition of DYRK1A by dNBC.

Subject Areas: Biology and Bio-materials

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Added On: 11/07/2011 08:22

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