Re-pairing DNA: Binding of a ruthenium phi complex to a double mismatch

DOI: 10.1039/D4SC01448K

Authors: Christine J. Cardin (University of Reading) , Tayler D. Prieto Otoya (University of Reading) , Kane T. Mcquaid (University of Reading) , Neil G. Paterson (Diamond Light Source) , David J. Cardin (University of Reading) , Andrew Kellett (Dublin City University)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Chemical Science

State: Published (Approved)
Published: May 2024

Abstract: We report a crystal structure at atomic resolution (0.9 Å) of a ruthenium complex bound to a consecutive DNA double mismatch, which results in a TA basepair with flipped out thymine, together with the formation of an adenine bulge. The structure shows a form of metalloinsertion interaction of the Λ-[Ru(phen)2phi]2+ (phi= 9,10-phenanthrenediimine) complex at the bulge site. The metal complex interacts with the DNA via the major groove, where specific interactions between the adenines of the DNA and the phen ligands of the complex are formed. One Δ-[Ru(phen)2phi]2+ complex interacts via the minor groove, which shows sandwiching of its phi ligand between the phi ligands of the other two ruthenium complexes, and no interaction of its phen ligands with DNA. To our knowledge, this binding model represents a new form of metalloinsertion in showing major rather than minor groove insertion.

Subject Areas: Biology and Bio-materials, Chemistry


Instruments: I03-Macromolecular Crystallography

Added On: 19/05/2024 21:07

Documents:
d4sc01448k.pdf

Discipline Tags:

Biochemistry Chemistry Structural biology Inorganic Chemistry Life Sciences & Biotech

Technical Tags:

Diffraction Macromolecular Crystallography (MX)