Poly(l-proline)-stabilized polypeptide nanostructures via Ring-Opening Polymerization-Induced Self-Assembly (ROPISA)

DOI: 10.1021/acsmacrolett.4c00400

Authors: Ernesto Tinajero-Díaz (RCSI University of Medicine and Health Sciences) , Nicola Judge (RCSI University of Medicine and Health Sciences) , Bo Li (RCSI University of Medicine and Health Sciences) , Thomas Leigh (RCSI University of Medicine and Health Sciences) , Robert D. Murphy (RCSI University of Medicine and Health Sciences) , Paul D. Topham (Aston University) , Matthew J. Derry (Aston University) , Andreas Heise (RCSI University of Medicine and Health Sciences; The SFI Advanced Materials and Bioengineering Research Centre)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Acs Macro Letters

State: Published (Approved)
Published: July 2024
Diamond Proposal Number(s): 33098

Abstract: Poly(proline) II helical motifs located at the protein–water interface stabilize the three-dimensional structures of natural proteins. Reported here is the first example of synthetic biomimetic poly(proline)-stabilized polypeptide nanostructures obtained by a straightforward ring-opening polymerization-induced self-assembly (ROPISA) process through consecutive N-carboxyanhydride (NCA) polymerization. It was found that the use of multifunctional 8-arm initiators is critical for the formation of nanoparticles. Worm-like micelles as well as spherical morphologies were obtained as confirmed by dynamic light scattering (DLS), transmission electron microscopy (TEM), and small angle X-ray scattering (SAXS). The loading of the nanostructures with dyes is demonstrated. This fast and open-vessel procedure gives access to amino acids-based nanomaterials with potential for applications in nanomedicine.

Subject Areas: Materials, Chemistry, Medicine


Instruments: I22-Small angle scattering & Diffraction

Added On: 31/07/2024 09:05

Documents:
tinajero-díaz-et-al-2024-poly(l-proline)-stabilized-polypeptide-nanostructures-via-ring-opening-polymerization-induced.pdf

Discipline Tags:

Drug Delivery Health & Wellbeing Biochemistry Chemistry Materials Science Nanoscience/Nanotechnology Polymer Science Life Sciences & Biotech

Technical Tags:

Scattering Small Angle X-ray Scattering (SAXS)