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New insights into the structure of the reaction centre from Blastochloris viridis: evolution in the laboratory

DOI: 10.1042/BJ20111540 DOI Help
PMID: 22054235 PMID Help

Authors: Aleksander W. Roszak (University of Glasgow) , Vladimira Moulisova (University of Glasgow) , Adhie D. P. Reksodipuro (University of Glasgow) , Alastair T. Gardiner (University of Glasgow) , Ritsuko Fujii (Osaka City University; Japan Science and Technology Agency (JST)) , Hideki Hashimoto (Osaka City University; Japan Science and Technology Agency (JST)) , Neil W. Isaacs (University of Glasgow) , Richard J. Cogdell (University of Glasgow)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Biochemical Journal

State: Published (Approved)
Published: November 2011
Diamond Proposal Number(s): 1229

Abstract: Newly determined crystal structures of the photosynthetic RC (reaction centre) from two substrains of the non-sulfur purple bacterium Blastochloris viridis strain DSM 133, together with analysis of their gene sequences, has revealed intraspecies evolutionary changes over a period of 14 years. Over 100 point mutations were identified between these two substrains in the four genes encoding the protein subunits of the RC, of which approximately one-fifth resulted in a total of 16 amino acid changes. The most interesting difference was in the M subunit where the change from a leucine residue to glycine in the carotenoid-binding pocket allowed NS5 (1,2-dihydroneurosporene) to adopt a more sterically favoured conformation, similar to the carotenoid conformation found in other related RCs. The results of the present study, together with a high rate of mutations in laboratory bacterial cultures described recently, suggest that bacteria evolve faster than has been generally recognized. The possibility that amino acid changes occur within protein sequences, without exhibiting any immediately observable phenotype, should be taken into account in studies that involve long-term continuous growth of pure bacterial cultures. The Blc. viridis RC is often studied with sophisticated biophysical techniques and changes such as those described here may well affect their outcome. In other words, there is a danger that laboratory-to-laboratory variation could well be due to different groups not realising that they are actually working with slightly different proteins. A way around this problem is suggested.

Journal Keywords: Blastochloris viridis; evolution; genetic drift; integral membrane pigment–protein complex; photosynthetic reaction centre; purple photosynthetic bacteria; reaction centre carotenoid; reaction centre polypeptide sequence

Diamond Keywords: Bacteria

Subject Areas: Biology and Bio-materials

Instruments: I02-Macromolecular Crystallography

Other Facilities: ID14-1, BM14 at ESRF

Added On: 08/11/2011 13:22

Discipline Tags:

Evolutionary science Structural biology Life Sciences & Biotech

Technical Tags:

Diffraction Macromolecular Crystallography (MX)