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Structure and Mechanism of the CMR Complex for CRISPR-Mediated Antiviral Immunity

DOI: 10.1016/j.molcel.2011.12.013 DOI Help
PMID: 22227115 PMID Help

Authors: Jing Zhang (University of St Andrews) , Christophe Rouillon (University of St Andrews) , Melina Kerou (University of St Andrews) , Judith Reeks (University of St Andrews) , Kim Brugger (University of Cambridge) , Shirley Graham (University of St Andrews) , Julia Reimann (Max Planck Institute for Terrestrial Microbiology) , Giuseppe Cannone (University of Edinburgh) , Huanting Liu (University of St Andrews) , Sonja-verena Albers (Max Planck Institute for Terrestrial Microbiology) , James H. Naismith (Biomedical Sciences Research Complex, University of St Andrews) , Laura Spagnolo (Institute of Structural Molecular Biology and Centre for Science at Extreme Conditions, University of Edinburgh) , Malcolm f. White (Biomedical Sciences Research Complex, University of St Andrews)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Molecular Cell

State: Published (Approved)
Published: January 2012

Abstract: The prokaryotic clusters of regularly interspaced palindromic repeats (CRISPR) system utilizes genomically encoded CRISPR RNA (crRNA), derived from invading viruses and incorporated into ribonucleoprotein complexes with CRISPR-associated (CAS) proteins, to target and degrade viral DNA or RNA on subsequent infection. RNA is targeted by the CMR complex. In Sulfolobus solfataricus, this complex is composed of seven CAS protein subunits (Cmr1-7) and carries a diverse “payload” of targeting crRNA. The crystal structure of Cmr7 and low-resolution structure of the complex are presented. S. solfataricus CMR cleaves RNA targets in an endonucleolytic reaction at UA dinucleotides. This activity is dependent on the 8 nt repeat-derived 5? sequence in the crRNA, but not on the presence of a protospacer-associated motif (PAM) in the target. Both target and guide RNAs can be cleaved, although a single molecule of guide RNA can support the degradation of multiple targets.

Journal Keywords: Archaeal; Base; Crystallography; X-Ray; Inverted; Macromolecular; Microscopy; Electron; Models; Molecular; Nucleic; Protein; Quaternary; Protein; Tertiary; Protein; RNA; RNA; Archaeal; Sulfolobus solfataricus

Subject Areas: Biology and Bio-materials

Instruments: I03-Macromolecular Crystallography