FolX from Pseudomonas aeruginosa is octameric in both crystal and solution

DOI: 10.1016/j.febslet.2012.03.031
PMID: 22575651

Authors: Mads Gabrielsen (University of Glasgow) , Katherine S. H. Beckham (University of Glasgow) , Richard J. Cogdell (University of Glasgow) , Olwyn Byron (University of Glasgow) , Andrew J. Roe (University of Glasgow)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Febs Letters , VOL 586 (8) , PAGES 1160-1165

State: Published (Approved)
Published: March 2012
Diamond Proposal Number(s): 6683

Abstract: Structural investigation of FolX from Pseudomonasaeruginosa; A combination of AUC and SAXS allows the X-ray structure to be ‘improved'; FolX is shown an octamer in both crystal and in solution; A network of 16 hydrogen bonds and 8 salt bridges provide octamer stability.

Journal Keywords: Bacterial; Catalytic; Circular; CFolX; 7,8-dihydroneopterin-triphosphate-epimerase; AUC; analytical ultracentrifugation; SAXS; small-angle X-ray scattering; SV; sedimentation velocity; SE; sedimentation equilibrium; Pteridine biosynthesis; X-ray structure

Diamond Keywords: Bacteria

Subject Areas: Biology and Bio-materials


Instruments: I03-Macromolecular Crystallography

Added On: 29/03/2012 16:37

Documents:
FEBS Letters - 2012 - Gabrielsen - FolX from Pseudomonas aeruginosa is octameric in both crystal and solution.pdf

Discipline Tags:

Pathogens Antibiotic Resistance Health & Wellbeing Structural biology Life Sciences & Biotech

Technical Tags:

Diffraction Macromolecular Crystallography (MX)