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Gating mechanisms for biological electron transfer: Integrating structure with biophysics reveals the nature of redox control in cytochrome P450 reductase and copper-dependent nitrite reductase

DOI: 10.1016/j.febslet.2011.07.003 DOI Help

Authors: Nicole G.h. Leferink (Manchester Interdisciplinary Biocentre, Manchester University, U.K.) , Christopher R. Pudney (Manchester Interdisciplinary Biocentre, Manchester University, U.K) , Sibylle Brenner (Manchester Interdisciplinary Biocentre, Manchester University, U.K) , Derren J. Heyes (Manchester Interdisciplinary Biocentre, Manchester University, U.K) , Robert R. Eady (Molecular Biophysics Group, Institute of Integrative Biology, University of Liverpool, U.K.) , S. Samar Hasnain (Molecular Biophysics Group, Institute of Integrative Biology, University of Liverpool, Liverpool, U.K.) , Sam Hay (Manchester Interdisciplinary Biocentre, Manchester University, U.K) , Stephen E.j. Rigby (Manchester Interdisciplinary Biocentre, Manchester University, U.K) , Nigel S. Scrutton (Manchester Interdisciplinary Biocentre, Manchester University, U.K)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Febs Letters , VOL 586 (5) , PAGES 578 - 584

State: Published (Approved)
Published: March 2012
Diamond Proposal Number(s): 7044

Abstract: Biological electron transfer is a fundamentally important reaction. Despite the apparent simplicity of these reactions (in that no bonds are made or broken), their experimental interrogation is often complicated because of adiabatic control exerted through associated chemical and conformational change. We have studied the nature of this control in several enzyme systems, cytochrome P450 reductase, methionine synthase reductase and copper-dependent nitrite reductase. Specifically, we review the evidence for conformational control in cytochrome P450 reductase and methionine synthase reductase and chemical control i.e. proton coupled electron transfer in nitrite reductase. This evidence has accrued through the use and integration of structural, spectroscopic and advanced kinetic methods. This integrated approach is shown to be powerful in dissecting control mechanisms for biological electron transfer and will likely find widespread application in the study of related biological redox systems.

Journal Keywords: Electron transfer; Gating; Proton coupled electron transfer; Conformationally controlled electron transfer

Subject Areas: Biology and Bio-materials, Chemistry, Environment


Instruments: I02-Macromolecular Crystallography , I03-Macromolecular Crystallography