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Conformational changes in IgE contribute to its uniquely slow dissociation rate from receptor Fc(Epsilon)RI.

DOI: 10.1038/nsmb.2044 DOI Help
PMID: 21516097 PMID Help

Authors: Mary D Holdom (King's College London, U.K) , Anna M Davies (King's College London, U.K.) , Joanne E Nettleship (University of Oxford, U.K.) , Sarah C Bagby (University Of Oxford, U.K) , Balvinder Dhaliwal (King's College London, U.K.) , Enrico Girardi (King's College London, U.K.) , James Hunt (King's College London, U.K.) , Hannah J Gould (King's College London, U.K.) , Andrew J Beavil (King's College London, U.K.) , James M Mcdonnell (King's College London, U.K.) , Ray J Owens (University of Oxford, U.K.) , Brian J Sutton (King's College London, U.K.)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Nature Structural & Molecular Biology , VOL 18 (5)

State: Published (Approved)
Published: December 2011
Diamond Proposal Number(s): 1220

Abstract: Among antibody classes, IgE has a uniquely slow dissociation rate from, and high affinity for, its cell surface receptor FcɛRI. We show the structural basis for these key determinants of the ability of IgE to mediate allergic hypersensitivity through the 3.4-Å-resolution crystal structure of human IgE-Fc (consisting of the Cɛ2, Cɛ3 and Cɛ4 domains) bound to the extracellular domains of the FcɛRI α chain. Comparison with the structure of free IgE-Fc (reported here at a resolution of 1.9 Å) shows that the antibody, which has a compact, bent structure before receptor engagement, becomes even more acutely bent in the complex. Thermodynamic analysis indicates that the interaction is entropically driven, which explains how the noncontacting Cɛ2 domains, in place of the flexible hinge region of IgG antibodies, contribute together with the conformational changes to the unique binding properties of IgE.

Journal Keywords: Binding; Humans; Immunoglobulin; Models; Molecular; Protein; Tertiary; Receptors; IgE; Thermodynamics

Subject Areas: Biology and Bio-materials


Instruments: I04-Macromolecular Crystallography

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