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Titanium phosphate glass microspheres for bone tissue engineering

DOI: 10.1016/j.actbio.2012.07.023 DOI Help

Authors: Nilay Lakhkar (University College London) , Jeong-hui Park (Dankook University, Republic of Korea) , Nicola J. Mordan (University College London, U.K.) , Vehid Salih (University College London, U.K.) , Ivan B. Wall (Dankook University, Republic of Korea) , Hae-won Kim (Dankook University, Republic of Korea) , Scott P. King (University of Warwick, U.K.) , John V. Hanna (University of Warwick, U.K.) , Richard Martin (Aston University) , Owen Addison (University of Birmingham) , Jonathan C. Knowles (University College London, U.K.) , J.f.w. Mosselmans (Diamond Light Source)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Acta Biomaterialia

State: Published (Approved)
Published: July 2012
Diamond Proposal Number(s): 5770

Abstract: We have demonstrated the successful production of titanium phosphate glass microspheres in the size range of ?10–200 ?m using an inexpensive, efficient, easily scalable process and assessed their use in bone tissue engineering applications. Glasses of the following compositions were prepared by melt-quench techniques: 0.5P2O5–0.4CaO–(0.1 ? x)Na2O–xTiO2, where x = 0.03, 0.05 and 0.07 mol fraction (denoted as Ti3, Ti5 and Ti7 respectively). Several characterization studies such as differential thermal analysis, degradation (performed using a novel time lapse imaging technique) and pH and ion release measurements revealed significant densification of the glass structure with increased incorporation of TiO2 in the glass from 3 to 5 mol.%, although further TiO2 incorporation up to 7 mol.% did not affect the glass structure to the same extent. Cell culture studies performed using MG63 cells over a 7-day period clearly showed the ability of the microspheres to provide a stable surface for cell attachment, growth and proliferation. Taken together, the results confirm that 5 mol.% TiO2 glass microspheres, on account of their relative ease of preparation and favourable biocompatibility, are worthy candidates for use as substrate materials in bone tissue engineering applications.

Journal Keywords: Titanium; Phosphate glass; Microsphere; Bone cells; Tissue engineering

Subject Areas: Biology and Bio-materials, Medicine


Instruments: I18-Microfocus Spectroscopy

Other Facilities: no