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The design of macromolecular crystallography diffraction experiments

DOI: 10.1107/S0907444911007608 DOI Help
PMID: 21460444 PMID Help

Authors: Gwyndaf Evans (Diamond Light Source) , Danny Axford (Diamond Light Source) , Robin L. Owen (Diamond Light Source)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Acta Crystallographica Section D , VOL 67 (4) , PAGES 261-270

State: Published (Approved)
Published: January 2011

Open Access Open Access

Abstract: The measurement of X-ray diffraction data from macro­molecular crystals for the purpose of structure determination is the convergence of two processes: the preparation of diffraction-quality crystal samples on the one hand and the construction and optimization of an X-ray beamline and end station on the other. Like sample preparation, a macromolecular crystallography beamline is geared to obtaining the best possible diffraction measurements from crystals provided by the synchrotron user. This paper describes the thoughts behind an experiment that fully exploits both the sample and the beamline and how these map into everyday decisions that users can and should make when visiting a beamline with their most precious crystals.

Journal Keywords: Convergence; Crystallography; Design; Molecular Crystals; Optimization; Sample Preparation; Synchrotron Radiation; Synchrotrons; X-Ray Diffraction

Subject Areas: Technique Development

Instruments: I24-Microfocus Macromolecular Crystallography