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Crystallization and preliminary X-ray analysis of Pac17 from the pacidamycin-biosynthetic cluster of Streptomyces coeruleorubidus
DOI:
10.1107/S1744309112029144
PMID:
22869135
Authors:
Daniel
Tromans
(John Innes Centre)
,
Clare
Stevenson
(John Innes Centre)
,
Rebecca J. M.
Goss
(University of East Anglia)
,
David
Lawson
(John Innes Centre)
Co-authored by industrial partner:
No
Type:
Journal Paper
Journal:
Acta Crystallographica Section F Structural Biology And Crystallization Communications
, VOL 68 (8)
, PAGES 971 - 974
State:
Published (Approved)
Published:
August 2012
Diamond Proposal Number(s):
1219
Abstract: Pac17 is an uncharacterized protein from the pacidamycin gene cluster of the soil bacterium Streptomyces coeruleorubidus. It is implicated in the biosynthesis of the core diaminobutyric acid residue of the antibiotic, although its precise role is uncertain at present. Given that pacidamycins inhibit translocase I of Pseudomonas aeruginosa, a clinically unexploited antibiotic target, they offer new hope in the search for antibacterial agents directed against this important pathogen. Crystals of Pac17 were grown by vapour diffusion and X-ray data were collected at a synchrotron to a resolution of 1.9 Å from a single crystal. The crystal belonged to space group C2, with unit-cell parameters a = 214.12, b = 70.88, c = 142.22 Å, [beta] = 92.96°. Preliminary analysis of these data suggests that the asymmetric unit consists of one Pac17 homotetramer, with an estimated solvent content of 49.0%.
Journal Keywords: Pac17; Streptomyces; Pacidamycin; Antibiotic Biosynthesis; Diaminobutyric Acid
Diamond Keywords: Bacteria
Subject Areas:
Biology and Bio-materials,
Chemistry,
Medicine
Instruments:
I02-Macromolecular Crystallography
Added On:
11/09/2012 09:34
Discipline Tags:
Pathogens
Antibiotic Resistance
Health & Wellbeing
Biochemistry
Chemistry
Structural biology
Drug Discovery
Life Sciences & Biotech
Technical Tags:
Diffraction
Macromolecular Crystallography (MX)