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Digital Ion Trap Mass Spectrometer for Probing the Structure of Biological Macromolecules by Gas Phase X-ray Scattering

DOI: 10.1021/ac802662d DOI Help
PMID: 19354287 PMID Help

Authors: Bryan Mccullough (The University of Manchester) , Andrew Entwistle (Shimadzu Research Laboratory (UK)) , Ikuo Konishi (Shimadzu Research Laboratory (Europe) Ltd) , Steve Buffey (Daresbury Lab) , Samar Hasnain (University of Liverpool) , Francesca Brancia (Shimadzu Research Laboratory (Europe) Ltd) , J. Guenter Grossmann (University of Liverpool) , Simon Gaskell (The University of Manchester)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Analytical Chemistry , VOL 81 (9) , PAGES 3392–3397

State: Published (Approved)
Published: April 2009

Abstract: Small-angle X-ray scattering is a technique for the characterization and structural analysis of a variety of materials including biological macromolecules and polymers. For the conformational analysis of proteins, the interaction between sample and X-rays is generally performed when the proteins are present in solution. Here a three-dimensional digital ion trap interfaced with a high intensity X-ray source is built to prove that X-ray scattering can be performed on ions isolated in gas-phase. Initial experiments on an unresolved ion population of multiply charged cytochrome C ions indicate that a small-angle X-ray scattering signal can be detected and that partial structural information can be extracted about the overall molecular structure of protein ions.

Subject Areas: Chemistry

Instruments: I22-Small angle scattering & Diffraction