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X-ray-excited optical luminescence of protein crystals: a new tool for studying radiation damage during diffraction data collection

DOI: 10.1107/S0907444912002946 DOI Help
PMID: 22525748 PMID Help

Authors: Robin L. Owen (Diamond Light Source) , Briony A. Yorke (University of Leeds) , Arwen Pearson (University of Leeds)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Acta Crystallographica Section D Biological Crystallography , VOL 68 (5) , PAGES 505 - 510

State: Published (Approved)
Published: May 2012
Diamond Proposal Number(s): 6386

Abstract: During X-ray irradiation protein crystals radiate energy in the form of small amounts of visible light. This is known as X-ray- excited optical luminescence (XEOL). The XEOL of several proteins and their constituent amino acids has been char- acterized using the microspectrophotometers at the Swiss Light Source and Diamond Light Source. XEOL arises primarily from aromatic amino acids, but the effects of local environment and quenching within a crystal mean that the XEOL spectrum of a crystal is not the simple sum of the spectra of its constituent parts. Upon repeated exposure to X-rays XEOL spectra decay non-uniformly, suggesting that XEOL is sensitive to site-speci\ufb01c radiation damage. However, rates of XEOL decay were found not to correlate to decays in diffracting power, making XEOL of limited use as a metric for radiation damage to protein crystals.

Journal Keywords: X-Ray-Excited Optical Luminescence; Radiation Damage; Macromolecular Crystallography

Subject Areas: Biology and Bio-materials


Instruments: I24-Microfocus Macromolecular Crystallography

Other Facilities: Swiss Light Source

Added On: 01/10/2012 21:23

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