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Expression, purification and crystallization of the Crimean–Congo haemorrhagic fever virus nucleocapsid protein

DOI: 10.1107/S1744309112009736 DOI Help
PMID: 22691790 PMID Help

Authors: S. D. Carter (University of Leeds, U.K.) , J. N. Barr (University of Leeds, U.K.) , T. A. Edwards (University of Leeds, U.K.)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Acta Crystallographica Section F Structural Biology And Crystallization Communications , VOL 68 (5) , PAGES 569 - 573

State: Published (Approved)
Published: May 2012
Diamond Proposal Number(s): 6386

Abstract: Crimean-Congo haemorrhagic fever virus (CCHFV) is a member of the Nairovirus genus within the Bunyaviridae family of segmented negative-sense RNA viruses. This paper describes the expression, purification and crystallization of full-length CCHFV nucleocapsid (N) protein and the collection of a 2.1 Å resolution X-ray diffraction data set using synchrotron radiation. Crystals of the CCHFV N protein belonged to space group C2, with unit-cell parameters a = 150.38, b = 72.06, c = 101.23 Å, = 110.70° and two molecules in the asymmetric unit. Circular-dichroism analysis provided insight into the secondary structure, whilst gel-filtration analysis revealed possible oligomeric states of the N protein. Structural determination is ongoing

Journal Keywords: Crimean-Congo Haemorrhagic Fever Virus; Cchfv Nucleocapsid (N) Protein; X-Ray Diffraction

Subject Areas: Biology and Bio-materials


Instruments: I02-Macromolecular Crystallography , I03-Macromolecular Crystallography , I04-1-Macromolecular Crystallography (fixed wavelength) , I04-Macromolecular Crystallography , I24-Microfocus Macromolecular Crystallography

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