Concentration of various trace elements in the rat retina and their distribution in different structures

DOI: 10.1039/c2mt20157g

Authors: Marta Ugarte (University of Manchester) , Geoffrey W Grime (Surrey Ion Beam Centre, University of Surrey, U.K.) , Gillian Lord (ICP-MS Facility, University of Surrey, U.K.) , Tina Geraki (Diamond Light Source) , Joanna Collingwood (Warwick University) , Mary Finnegan (University of Warwick) , Hannah Farnfield (ICP-MS Facility, University of Surrey, U.K.) , Michael Merchant (Surrey Ion Beam Centre, University of Surrey, U.K.) , Melanie Bailey (Surrey Ion Beam Centre, University of Surrey, U.K.) , N. I. Ward (ICP-MS Facility, University of Surrey, U.K.) , Peter Foster (University of Manchester, U.K.) , Paul N Bishop (Centre for Advanced Discovery and Experimental Therapeutics, University of Manchester, U.K.) , Neville N Osborne (Instituto Oftalmológico Fernández-Vega, Spain)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Metallomics , VOL 12 (4) , PAGES 1245-1254

State: Published (Approved)
Published: October 2012
Diamond Proposal Number(s): 7454

Abstract: Inductively coupled plasma mass spectrometry (ICP-MS) was used to quantify the total amount of trace elements in retina from adult male Sprague-Dawley rats (n = 6). Concentration of trace elements within individual retinal areas in frozen sections of the fellow eye was established with the use of two methodologies: (1) particle-induced X-ray emission (PIXE) in combination with 3D depth profiling with Rutherford backscattering spectrometry (RBS) and (2) synchrotron X-ray fluorescence (SXRF) microscopy. The most abundant metal in the retina was zinc, followed by iron and copper. Nickel, manganese, chromium, cobalt, selenium and cadmium were present in very small amounts. The PIXE and SXRF analysis yielded a non-homogenous pattern distribution of metals in the retina. Relatively high levels of zinc were found in the inner part of the photoreceptor inner segments (RIS)/outer limiting membrane (OLM), inner nuclear layer and plexiform layers. Iron was found to accumulate in the retinal pigment epithelium/choroid layer and RIS/OLM. Copper in turn, was localised primarily in the RIS/OLM and plexiform layers. The trace elements iron, copper, and zinc exist in different amounts and locations in the rat retina.

Journal Keywords: Iron; Zinc; Copper; Retina; Icp-Ms; Pixe; Synchrotron

Subject Areas: Biology and Bio-materials


Instruments: I18-Microfocus Spectroscopy