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Measuring protein dynamics with ultrafast two-dimensional infrared spectroscopy

DOI: 10.1088/0957-0233/23/6/062001 DOI Help

Authors: Katrin Adamczyk (University of Strathclyde) , Marco Candelaresi (University of Strathclyde) , Kirsty Robb (Strathclyde Institute of Pharmacy and Biomedical Sciences) , Andrea Gumiero (Diamond Light Source) , Martin A. Walsh (Diamond Light Source) , Anthony W. Parker (Central Laser Facility, Research Complex at Harwell) , Paul A. Hoskisson (Strathclyde Institute of Pharmacy and Biomedical Sciences) , Nicholas P. Tucker (Strathclyde Institute of Pharmacy and Biomedical Sciences) , Neil T. Hunt (University of Strathclyde)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Measurement Science And Technology , VOL 23 (6)

State: Published (Approved)
Published: June 2012

Abstract: Recent advances in the methodology and application of ultrafast two-dimensional infrared (2D-IR) spectroscopy to biomolecular systems are reviewed. A description of the 2D-IR technique and the molecular contributions to the observed spectra are presented followed by a discussion of recent literature relating to the use of 2D-IR and associated approaches for measuring protein dynamics. In particular, these include the use of diatomic ligand groups for measuring haem protein dynamics, isotopic labelling strategies and the use of vibrational probe groups. The final section reports on the current state of the art regarding the use of 2D-IR methods to provide insights into biological reaction mechanisms.

Journal Keywords: Absorption Spectroscopy; Biochemical Reaction Kinetics; Infrared Spectra; Ligands; Measuring Methods; Proteins; Reviews; Two-Dimensional Calculations

Subject Areas: Biology and Bio-materials, Technique Development

Technical Areas:

Added On: 08/10/2012 14:25

Discipline Tags:

Technique Development - Life Sciences & Biotech Life Sciences & Biotech

Technical Tags: