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'Seeding’ with protease to optimize protein crystallization conditions in in situ proteolysis
DOI:
10.1107/S174430911201161X
PMID:
22691798
Authors:
Jinguang
Huang
(China Agricultural University; Qingdao Agricultural University)
,
Yanmei
Gong
(China Agricultural University)
,
Dan
Huang
(China Agricultural University)
,
Lesley
Haire
(MRC-NIMR)
,
Junfeng
Liu
(China Agricultural University)
,
Youliang
Peng
(China Agricultural University)
Co-authored by industrial partner:
No
Type:
Journal Paper
Journal:
Acta Crystallographica Section F Structural Biology And Crystallization Communications
, VOL 68 (5)
, PAGES 606 - 609
State:
Published (Approved)
Published:
May 2012
Abstract: In situ proteolysis is one of the most effective rescue strategies for protein crystallization, and optimization of the ratio between the protein and the protease is one of the key steps in the process. Seeding is a very powerful tool to optimize crystallization conditions and can be performed by most crystallization robots. Addition of protease instead of seed stock using a robot can be used to optimize the concentration of protease in in situ proteolysis experiments and has been successfully tested using two proteins.
Subject Areas:
Biology and Bio-materials
Instruments:
I02-Macromolecular Crystallography
,
I04-Macromolecular Crystallography
Other Facilities: BL-17U at Shanghai Synchrotron Research Facility (SSRF); 3W1A, 1W2B at Beijing Synchrotron Research Facility (BSRF)
Added On:
09/11/2012 18:18
Discipline Tags:
Structural biology
Life Sciences & Biotech
Technical Tags:
Diffraction
Macromolecular Crystallography (MX)