‘Seeding’ with protease to optimize protein crystallization conditions in in situ proteolysis

DOI: 10.1107/S174430911201161X
PMID: 22691798

Authors: Jinguang Huang (State Key Laboratory of Agribiotechnology and MOA Key Laboratory of Plant Pathology, China) , Yanmei Gong (State Key Laboratory of Agribiotechnology and MOA Key Laboratory of Plant Pathology, China) , Dan Huang (State Key Laboratory of Agribiotechnology and MOA Key Laboratory of Plant Pathology, China) , Lesley Haire (Division of Molecular Structure, MRC-NIMR, England) , Junfeng Liu (State Key Laboratory of Agribiotechnology and MOA Key Laboratory of Plant Pathology, China) , Youliang Peng (State Key Laboratory of Agribiotechnology and MOA Key Laboratory of Plant Pathology, China)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Acta Crystallographica Section F Structural Biology And Crystallization Communications , VOL 68 (5) , PAGES 606 - 609

State: Published (Approved)
Published: May 2012

Abstract: In situ proteolysis is one of the most effective rescue strategies for protein crystallization, and optimization of the ratio between the protein and the protease is one of the key steps in the process. Seeding is a very powerful tool to optimize crystallization conditions and can be performed by most crystallization robots. Addition of protease instead of seed stock using a robot can be used to optimize the concentration of protease in in situ proteolysis experiments and has been successfully tested using two proteins.

Subject Areas: Biology and Bio-materials


Instruments: I02-Macromolecular Crystallography , I04-Macromolecular Crystallography

Other Facilities: Shanghai Synchrotron Research Facility (SSRF); Beijing Synchrotron Research Facility (BSRF)

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