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How nature can exploit nonspecific catalytic and carbohydrate binding modules to create enzymatic specificity

DOI: 10.1073/pnas.1212034109 DOI Help
PMID: 23213210 PMID Help

Authors: Fiona Cuskin (Newcastle University) , James E. Flint (Newcastle University) , Tracey M. Gloster (University of St Andrews) , Carl Morland (Newcastle University) , Arnaud Basle (Newcastle University) , Bernard Henrissat (Aix-Marseille Université) , Pedro M. Coutinho (Aix-Marseille Université) , Andrea Strazzulli (Consiglio Nazionale delle Ricerche) , Alexandra S. Solovyova (Newcastle University) , Gideon J. Davies (University of York) , Harry J. Gilbert (The University of Georgia)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Proceedings Of The National Academy Of Sciences

State: Published (Approved)
Published: December 2012

Abstract: Noncatalytic carbohydrate binding modules (CBMs) are components of glycoside hydrolases that attack generally inaccessible substrates. CBMs mediate a two- to fivefold elevation in the activity of endo-acting enzymes, likely through increasing the concentration of the appended enzymes in the vicinity of the substrate. The function of CBMs appended to exo-acting glycoside hydrolases is unclear because their typical endo-binding mode would not fulfill a targeting role. Here we show that the Bacillus subtilis exo-acting ?-fructosidase SacC, which specifically hydrolyses levan, contains the founding member of CBM family 66 (CBM66). The SacC-derived CBM66 (BsCBM66) targets the terminal fructosides of the major fructans found in nature. The crystal structure of BsCBM66 in complex with ligands reveals extensive interactions with the terminal fructose moiety (Fru-3) of levantriose but only limited hydrophobic contacts with Fru-2, explaining why the CBM displays broad specificity. Removal of BsCBM66 from SacC results in a ?100-fold reduction in activity against levan. The truncated enzyme functions as a nonspecific ?-fructosidase displaying similar activity against ?-2,1– and ?-2,6–linked fructans and their respective fructooligosaccharides. Conversely, appending BsCBM66 to BT3082, a nonspecific ?-fructosidase from Bacteroides thetaiotaomicron, confers exolevanase activity on the enzyme. We propose that BsCBM66 confers specificity for levan, a branched fructan, through an “avidity” mechanism in which the CBM and the catalytic module target the termini of different branches of the same polysaccharide molecule. This report identifies a unique mechanism by which CBMs modulate enzyme function, and shows how specificity can be tailored by integrating nonspecific catalytic and binding modules into a single enzyme.

Journal Keywords: Bacteroides; Biofuels; Calorimetry; Carbohydrates; Catalysis; Crystallography; X-Ray; Enzymes; Fructans; Glycoside; Hydrophobic; Kinetics; Lectins; Ligands; Models; Chemical; Oligosaccharides; Polysaccharides; Protein; Tertiary

Subject Areas: Biology and Bio-materials

Instruments: I02-Macromolecular Crystallography , I03-Macromolecular Crystallography , I04-Macromolecular Crystallography

Other Facilities: ESRF

Added On: 10/12/2012 08:41

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