Expression, purification and crystallization of the ectodomain of the envelope glycoprotein E2 from Bovine viral diarrhoea virus

DOI: 10.1107/S1744309112049184
PMID: 23295482

Authors: Oleg Iourin (Wellcome Trust Centre for Human Genetics, University of Oxford) , Karl Harlos (Wellcome Trust Centre for Human Genetics, University of Oxford) , Kamel El Omari (Wellcome Trust Centre for Human Genetics, University of Oxford) , Weixian Lu (Wellcome Trust Centre for Human Genetics, University of Oxford) , Jan Kadlec (Wellcome Trust Centre for Human Genetics, University of Oxford) , Munir Iqbal (Institute for Animal Health) , Christoph Meier (Wellcome Trust Centre for Human Genetics, University of Oxford) , Andrew Palmer (University of Reading) , Ian Jones (University of Reading) , Carole Thomas (The Royal Veterinary College) , Joe Brownlie (The Royal Veterinary College) , Jonathan M. Grimes (Wellcome Trust Centre for Human Genetics, University of Oxford; Diamond Light Source) , David I. Stuart (Diamond Light Source)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Acta Crystallographica Section F Structural Biology And Crystallization Communications , VOL 69 , PAGES 35 - 38

State: Published (Approved)
Published: January 2013

Abstract: Bovine viral diarrhoea virus (BVDV) is an economically important animal pathogen which is closely related to Hepatitis C virus. Of the structural proteins, the envelope glycoprotein E2 of BVDV is the major antigen which induces neutralizing antibodies; thus, BVDV E2 is considered as an ideal target for use in subunit vaccines. Here, the expression, purification of wild-type and mutant forms of the ectodomain of BVDV E2 and subsequent crystallization and data collection of two crystal forms grown at low and neutral pH are reported. Native and multiple-wavelength anomalous dispersion (MAD) data sets have been collected and structure determination is in progress.

Journal Keywords: Cattle; Crystallization; Crystals; Dispersions; Wavelengths; X-Ray Diffraction

Diamond Keywords: Viruses; Bovine Viral Diarrhoea (BVD)

Subject Areas: Biology and Bio-materials


Instruments: I02-Macromolecular Crystallography , I03-Macromolecular Crystallography , I24-Microfocus Macromolecular Crystallography

Added On: 07/01/2013 09:59

Discipline Tags:

Pathogens Agriculture & Fisheries Structural biology Life Sciences & Biotech Veterinary Medicine

Technical Tags:

Diffraction Macromolecular Crystallography (MX)