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Piperidinols That Show Anti-Tubercular Activity as Inhibitors of Arylamine N-Acetyltransferase: An Essential Enzyme for Mycobacterial Survival Inside Macrophages

DOI: 10.1371/journal.pone.0052790 DOI Help
PMID: 23285185 PMID Help

Authors: Areej Abuhammad (University of Oxford) , Elizabeth Fullam (University of Birmingham) , Ed Lowe (University of Oxford) , David Staunton , Akane Kawamura , Isaac Westwood (Institute of Cancer Research) , Sanjib Bhakta , Alun Christopher Garner , David L. Wilson , Peter T. Seden , Stephen Davies , Angela J. Russell , Elspeth Garman (University of Oxford) , Edith Sim (University of Oxford) , Anil Kumar Tyagi
Co-authored by industrial partner: No

Type: Journal Paper
Journal: PLoS ONE , VOL 7 (12)

State: Published (Approved)
Published: December 2012

Open Access Open Access

Abstract: Latent M. tuberculosis infection presents one of the major obstacles in the global eradication of tuberculosis (TB). Cholesterol plays a critical role in the persistence of M. tuberculosis within the macrophage during latent infection. Catabolism of cholesterol contributes to the pool of propionyl-CoA, a precursor that is incorporated into cell-wall lipids. Arylamine N-acetyltransferase (NAT) is encoded within a gene cluster that is involved in the cholesterol sterol-ring degradation and is essential for intracellular survival. The ability of the NAT from M. tuberculosis (TBNAT) to utilise propionyl-CoA links it to the cholesterol-catabolism pathway. Deleting the nat gene or inhibiting the NAT enzyme prevents intracellular survival and results in depletion of cell-wall lipids. TBNAT has been investigated as a potential target for TB therapies. From a previous high-throughput screen, 3-benzoyl-4-phenyl-1-methylpiperidinol was identified as a selective inhibitor of prokaryotic NAT that exhibited antimycobacterial activity. The compound resulted in time-dependent irreversible inhibition of the NAT activity when tested against NAT from M. marinum (MMNAT). To further evaluate the antimycobacterial activity and the NAT inhibition of this compound, four piperidinol analogues were tested. All five compounds exert potent antimycobacterial activity against M. tuberculosis with MIC values of 2.3–16.9 µM. Treatment of the MMNAT enzyme with this set of inhibitors resulted in an irreversible time-dependent inhibition of NAT activity. Here we investigate the mechanism of NAT inhibition by studying protein-ligand interactions using mass spectrometry in combination with enzyme analysis and structure determination. We propose a covalent mechanism of NAT inhibition that involves the formation of a reactive intermediate and selective cysteine residue modification. These piperidinols present a unique class of antimycobacterial compounds that have a novel mode of action different from known anti-tubercular drugs.

Journal Keywords: Antitubercular; Arylamine; Catalytic; Cell; Dose-Response; Drug ; Enzyme; Enzyme; Humans ; Macrophages ; Mice ; Mycobacterium ; Piperidines ; Protein Conformation

Subject Areas: Biology and Bio-materials


Instruments: I04-Macromolecular Crystallography

Added On: 08/01/2013 08:14

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