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SAXS Study of Sterically Stabilized Lipid Nanocarriers Functionalized by DNA

DOI: 10.1088/1742-6596/351/1/012004 DOI Help

Authors: Borislav Angelov (Institute of Macromolecular Chemistry, ASCR) , Angelina Angelova (CNRS UMR8612, Univ Paris Sud) , Sergey Filippov (Institute of Macromolecular Chemistry, ASCR) , Göran Karlsson (Uppsala University) , Nicholas Terrill (Diamond Light Source) , Sylviane Lesieur (CNRS UMR8612, Univ Paris Sud) , Petr Stepanek (Institute of Macromolecular Chemistry, ASCR)
Co-authored by industrial partner: No

Type: Conference Paper
Conference: SANS-YuMO User Meeting at the Start-up of Scientific Experiments on the IBR-2M Reactor: Devoted to the 75th anniversary of Yu M Ostanevich's birth 27–30 May 2011, Dubna, Russia
Peer Reviewed: Yes

State: Published (Approved)
Published: March 2012
Diamond Proposal Number(s): 3313

Abstract: The structure of novel spontaneously self-assembled plasmid DNA/lipid complexes is investigated by means of synchrotron radiation small-angle X-ray scattering (SAXS) and Cryo-TEM imaging. Liquid crystalline (LC) hydrated lipid systems are prepared using the non-ionic lipids monoolein and DOPE-PEG2000 and the cationic amphiphile CTAB. The employed plasmid DNA (pDNA) is encoding for the human protein brain-derived neurotrophic factor (BDNF). A coexistence of nanoparticulate objects with different LC inner organizations is established. A transition from bicontinuous membrane sponges, cubosome intermediates and unilamelar liposomes to multilamellar vesicles, functionalized by pDNA, is favoured upon binding and compaction of pBDNF onto the cationic PEGylated lipid nanocarriers. The obtained sterically stabilized multicompartment nanoobjects, with confined supercoiled plasmid DNA (pBDNF), are important in the context of multicompartment lipid nanocarriers of interest for gene therapy of neurodegenerative diseases.

Journal Keywords: Brain; Complexes; Dna; Gene Therapy; Human Populations; Lipids; Liposomes; Liquids; Membranes; Nervous System Diseases; Proteins; Small Angle Scattering; Synchrotron Radiation; X-Ray Diffraction

Subject Areas: Physics, Chemistry


Instruments: I22-Small angle scattering & Diffraction