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Structure determination from a single high-pressure-frozen virus crystal

DOI: 10.1107/S090744491204543X DOI Help
PMID: 23385466 PMID Help

Authors: Anja Burkhardt (Deutsches Elektronen-Synchrotron (DESY)) , Armin Wagner (Diamond Light Source) , Martin Warmer (Heinrich Pette Institute, Leibniz Institute for Experimental Virology) , Rudolph Reimer (Heinrich Pette Institute, Leibniz Institute for Experimental Virology) , Heinrich Hohenberg (Heinrich Pette Institute, Leibniz Institute for Experimental Virology) , Jingshan Ren (University of Oxford) , Elizabeth E. Fry (University of Oxford) , David I. Stuart (University of Oxford; Diamond Light Source) , Alke Meents (Deutsches Elektronen-Synchrotron (DESY))
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Acta Crystallographica Section D Biological Crystallography , VOL 69 , PAGES 308 - 312

State: Published (Approved)
Published: February 2013

Abstract: Successful cryogenic X-ray structure determination from a single high-pressure-frozen bovine enterovirus 2 crystal is reported. The presented high-pressure-freezing procedure is based on a commercially available device and allows the cryocooling of macromolecular crystals directly in their mother liquor without the time- and crystal-consuming search for optimal cryoconditions. The method is generally applicable and will allow cryogenic data collection from all types of macromolecular crystals.

Journal Keywords: Virology; Cryocooling; High-Pressure-Freezing

Subject Areas: Technique Development, Biology and Bio-materials


Instruments: I24-Microfocus Macromolecular Crystallography