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Promoting crystallisation of the Salmonella enteritidis fimbriae 14 pilin SefD using deuterium oxide

DOI: 10.1016/j.bbrc.2012.03.136 DOI Help
PMID: 22497887 PMID Help

Authors: Bing Liu (Imperial College London) , James Garnett (Imperial College London) , Wei-chao Lee (Imperial College London) , Jing Lin (Imperial College London) , Paula Salgado (Imperial College London) , Jonathan Taylor (Imperial College London) , Yingqi Xu (Imperial College London) , Sebastian Lambert (Imperial College London) , Ernesto Cota (Imperial College London) , Steve Matthews (Imperial College London)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Biochemical And Biophysical Research Communications , VOL 421 (2) , PAGES 208 - 213

State: Published (Approved)
Published: April 2012
Diamond Proposal Number(s): 7299

Abstract: The use of heavy water (D2O) as a solvent is commonplace in many spectroscopic techniques for the study of biological macromolecules. A significant deuterium isotope effect exists where hydrogen-bonding is important, such as in protein stability, dynamics and assembly. Here we illustrate the use of D2O in additive screening for the production of reproducible diffraction-quality crystals for the Salmonella enteritidis fimbriae 14 (SEF14) putative tip adhesin, SefD.

Journal Keywords: Crystallization; Crystallography; X-Ray; Deuterium; Fimbriae; Fimbriae; Bacterial; Protein; Tertiary; Salmonella enteritidis

Subject Areas: Biology and Bio-materials, Technique Development, Medicine

Instruments: I24-Microfocus Macromolecular Crystallography

Other Facilities: No