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Self-Assembly and Conformational Heterogeneity of the AXH Domain of Ataxin-1: An Unusual Example of a Chameleon Fold
DOI:
10.1016/j.bpj.2013.01.048
PMID:
23528090
Authors:
Cesira
De chiara
(Medical Research Council National Institute for Medical Research London)
,
Martin
Rees
(King's College London)
,
Raj p.
Menon
(Medical Research Council National Institute for Medical Research London)
,
Kris
Pauwels
(Medical Research Council National Institute for Medical Research London)
,
Ceri
Lawrence
(Medical Research Council National Institute for Medical Research London)
,
Petr v.
Konarev
(European Molecular Biology Laboratory)
,
Dmitri
Svergun
(European Molecular Biology Laboratory)
,
Stephen R.
Martin
(Medical Research Council National Institute for Medical Research London)
,
Yu Wai
Chen
(King's College London)
,
Annalisa
Pastore
(Medical Research Council National Institute for Medical Research London)
Co-authored by industrial partner:
No
Type:
Journal Paper
Journal:
Biophysical Journal
, VOL 104 (6)
, PAGES 1304 - 1313
State:
Published (Approved)
Published:
March 2013

Abstract: Ataxin-1 is a human protein responsible for spinocerebellar ataxia type 1, a hereditary disease associated with protein aggregation and misfolding. Essential for ataxin-1 aggregation is the anomalous expansion of a polyglutamine tract near the protein N-terminus, but the sequence-wise distant AXH domain modulates and contributes to the process. The AXH domain is also involved in the nonpathologic functions of the protein, including a variety of intermolecular interactions with other cellular partners. The domain forms a globular dimer in solution and displays a dimer of dimers arrangement in the crystal asymmetric unit. Here, we have characterized the domain further by studying its behavior in the crystal and in solution. We solved two new structures of the domain crystallized under different conditions that confirm an inherent plasticity of the AXH fold. In solution, the domain is present as a complex equilibrium mixture of monomeric, dimeric, and higher molecular weight species. This behavior, together with the tendency of the AXH fold to be trapped in local conformations, and the multiplicity of protomer interfaces, makes the AXH domain an unusual example of a chameleon protein whose properties bear potential relevance for the aggregation properties of ataxin-1 and thus for disease.
Journal Keywords: Models; Molecular; Mutation; Nerve; Nuclear; Protein; Quaternary; Protein; Tertiary; Scattering; Small; Solutions; X-Ray Diffraction
Subject Areas:
Biology and Bio-materials,
Physics,
Medicine
Instruments:
I02-Macromolecular Crystallography
,
I04-1-Macromolecular Crystallography (fixed wavelength)
Other Facilities: DESY