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Preferred orientation in an angled intercalation site of a chloro-substituted  -[Ru(TAP)2(dppz)]2+ complex bound to d(TCGGCGCCGA)2

DOI: 10.1098/rsta.2012.0525 DOI Help
PMID: 23776304 PMID Help

Authors: James P. Hall (University of Reading) , Hanna Beer (University of Reading) , Katrin Buchner (University of Reading) , David Cardin (Chemistry Department, University of Reading) , Christine J Cardin (University of Reading)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Philosophical Transactions Of The Royal Society A: Mathematical, Physical And Engineering Sciences , VOL 371 (1995) , PAGES 20120525 - 20120525

State: Published (Approved)
Published: June 2013

Abstract: The crystal structure of the ruthenium DNA ‘light-switch’ complex Λ-[Ru(TAP)2(11-Cl-dppz)]2+ (TAP=tetraazaphenanthrene, dppz=dipyrido[3,2-a′:2′,3′-c]phenazine) bound to the oligonucleotide duplex d(TCGGCGCCGA)2 is reported. The synthesis of the racemic ruthenium complex is described for the first time, and the racemate was used in this study. The crystal structure, at atomic resolution (1.0 Å), shows one ligand as a wedge in the minor groove, resulting in the 51° kinking of the double helix, as with the parent Λ-[Ru(TAP)2(dppz)]2+. Each complex binds to one duplex by intercalation of the dppz ligand and also by semi-intercalation of one of the orthogonal TAP ligands into a second symmetrically equivalent duplex. The 11-chloro substituent binds with the major component (66%) oriented with the 11-chloro substituent on the purine side of the terminal step of the duplex.

Journal Keywords: Chlorine; Intercalating; Models; Chemical; Models; Molecular; Nucleic; Oligonucleotides; Ruthenium

Subject Areas: Biology and Bio-materials

Instruments: I02-Macromolecular Crystallography