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Caenorhabditis elegans centriolar protein SAS-6 forms a spiral that is consistent with imparting a ninefold symmetry

DOI: 10.1073/pnas.1302721110 DOI Help
PMID: 23798409 PMID Help

Authors: Manuel Hilbert (Paul Scherrer Institut) , Michele C. Erat (University of Oxford) , Virginie Hachet (Swiss Federal Institute of Technology (EPFL)) , Paul Guichard (Swiss Federal Institute of Technology (EPFL)) , I. D. Blank (Paul Scherrer Institut) , Isabelle Fluckiger (Swiss Federal Institute of Technology (EPFL)) , Leanne Slater (University of Oxford) , Edward D. Lowe (University of Oxford) , Georgios N. Hatzopoulos (University of Oxford) , Michel O. Steinmetz (Paul Scherrer Institute) , Pierre Gonczy (Swiss Federal Institute of Technology (EPFL)) , Ioannis Vakonakis (University of Oxford)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Proceedings Of The National Academy Of Sciences

State: Published (Approved)
Published: June 2013

Abstract: Centrioles are evolutionary conserved organelles that give rise to cilia and flagella as well as centrosomes. Centrioles display a characteristic ninefold symmetry imposed by the spindle assembly abnormal protein 6 (SAS-6) family. SAS-6 from Chlamydomonas reinhardtii and Danio rerio was shown to form ninefold symmetric, ring-shaped oligomers in vitro that were similar to the cartwheels observed in vivo during early steps of centriole assembly in most species. Here, we report crystallographic and EM analyses showing that, instead, Caenorhabotis elegans SAS-6 self-assembles into a spiral arrangement. Remarkably, we find that this spiral arrangement is also consistent with ninefold symmetry, suggesting that two distinct SAS-6 oligomerization architectures can direct the same output symmetry. Sequence analysis suggests that SAS-6 spirals are restricted to specific nematodes. This oligomeric arrangement may provide a structural basis for the presence of a central tube instead of a cartwheel during centriole assembly in these species.

Journal Keywords: Caenorhabditis; Caenorhabditis; Cell; Crystallography; X-Ray; Microscopy; Electron; Models; Molecular; Protein Conformation

Subject Areas: Biology and Bio-materials

Instruments: I02-Macromolecular Crystallography , I04-1-Macromolecular Crystallography (fixed wavelength) , I24-Microfocus Macromolecular Crystallography

Other Facilities: ESRF

Added On: 01/07/2013 13:11

Discipline Tags:

Structural biology Life Sciences & Biotech

Technical Tags:

Diffraction Macromolecular Crystallography (MX)