Structural basis for native agonist and synthetic inhibitor recognition by the Pseudomonas aeruginosa quorum sensing regulator PqsR (MvfR)

DOI: 10.1371/journal.ppat.1003508
PMID: 23935486

Authors: Aravindan Ilangovan (University of Nottingham) , Matthew Fletcher (University of Nottingham) , Giordano Rampioni (University of Nottingham) , Christian Pustelny (University of Nottingham) , Kendra Rumbaugh (University of Texas) , Stephan Heeb (University of Nottingham) , Miguel Cámara (University of Nottingham) , Alex Truman (University of Nottingham) , Siri Ram Chhabra (University of Nottingham) , Jonas Emsley (University of Nottingham) , Paul Williams (University of Nottingham)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Plos Pathogens , VOL 9 (7)

State: Published (Approved)
Published: July 2013
Diamond Proposal Number(s): 6388

Abstract: Bacterial populations co-ordinate gene expression collectively through quorum sensing (QS), a cell-to-cell communication mechanism employing diffusible signal molecules. The LysR-type transcriptional regulator (LTTR) protein PqsR (MvfR) is a key component of alkyl-quinolone (AQ)-dependent QS in Pseudomonas aeruginosa. PqsR is activated by 2-alkyl-4-quinolones including the Pseudomonas quinolone signal (PQS; 2-heptyl-3-hydroxy-4(1H)-quinolone), its precursor 2-heptyl-4-hydroxyquinoline (HHQ) and their C9 congeners, 2-nonyl-3-hydroxy-4(1H)-quinolone (C9-PQS) and 2-nonyl-4-hydroxyquinoline (NHQ)

Journal Keywords: Anti-Bacterial; Bacterial; Binding; Biofilms; Drug; Gene; Bacterial; Ligands; Mutant; Peptide; Protein; Pseudomonas; Quinolones; Quorum; Recombinant; Signal; Structure-Activity; Transcription; Virulence

Diamond Keywords: Bacteria

Subject Areas: Biology and Bio-materials, Medicine


Instruments: I02-Macromolecular Crystallography , I04-Macromolecular Crystallography

Added On: 30/07/2013 09:49

Documents:
file-3.pdf

Discipline Tags:

Pathogens Antibiotic Resistance Infectious Diseases Health & Wellbeing Structural biology Drug Discovery Life Sciences & Biotech

Technical Tags:

Diffraction Macromolecular Crystallography (MX)