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Conformational flexibility and molecular interactions of an archaeal homologue of the Shwachman-Bodian-Diamond syndrome protein

DOI: 10.1186/1472-6807-9-32 DOI Help
PMID: 19454024 PMID Help

Authors: Chyan Ng (MRC LMB) , David Waterman (Diamond Light Source) , Eugene Koonin (NCBI, NIH) , Alison Walters (University of York) , James Chong (University of York) , Misha Isupov (University of Exeter) , Andrey Lebedev (University of York) , David Bunka (University of Leeds) , Miguel Ortiz-Lombardía (Architecture et Fonction des Macromolécules Biologiques, CNRS, Universités d'Aix-Marseille) , Fred Antson (University of York)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: BMC Structural Biology , VOL 9 , PAGES 32

State: Published (Approved)
Published: May 2009

Abstract: Background: Defects in the human Shwachman-Bodian-Diamond syndrome (SBDS) protein-coding gene lead to the autosomal recessive disorder characterised by bone marrow dysfunction, exocrine pancreatic insufficiency and skeletal abnormalities. This protein is highly conserved in eukaryotes and archaea but is not found in bacteria. Although genomic and biophysical studies have suggested involvement of this protein in RNA metabolism and in ribosome biogenesis, its interacting partners remain largely unknown.Results: We determined the crystal structure of the SBDS orthologue from Methanothermobacter thermautotrophicus (mthSBDS). This structure shows that SBDS proteins are highly flexible, with the N-terminal FYSH domain and the C-terminal ferredoxin-like domain capable of undergoing substantial rotational adjustments with respect to the central domain. Affinity chromatography identified several proteins from the large ribosomal subunit as possible interacting partners of mthSBDS. Moreover, SELEX (Systematic Evolution of Ligands by EXponential enrichment) experiments, combined with electrophoretic mobility shift assays (EMSA) suggest that mthSBDS does not interact with RNA molecules in a sequence specific manner.Conclusion: It is suggested that functional interactions of SBDS proteins with their partners could be facilitated by rotational adjustments of the N-terminal and the C-terminal domains with respect to the central domain. Examination of the SBDS protein structure and domain movements together with its possible interaction with large ribosomal subunit proteins suggest that these proteins could participate in ribosome function.

Subject Areas: Biology and Bio-materials

Facility: ESRF