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Structure of a bifunctional alcohol dehydrogenase involved in bioethanol generation in Geobacillus thermoglucosidasius

DOI: 10.1107/S0907444913020349 DOI Help
PMID: 24100328 PMID Help

Authors: Jonathan Extance (University of Bath) , Susan Crennell (University of Bath) , Kirstin Eley (TMO Renewables Ltd) , Roger Cripps (TMO Renewables Ltd) , David Hough (University of Bath) , Michael Danson (University of Bath)
Co-authored by industrial partner: Yes

Type: Journal Paper
Journal: Acta Crystallographica Section D Biological Crystallography , VOL 69 , PAGES 2104 - 2115

State: Published (Approved)
Published: October 2013
Diamond Proposal Number(s): 1226

Abstract: Bifunctional alcohol/aldehyde dehydrogenase (ADHE) enzymes are found within many fermentative microorganisms. They catalyse the conversion of an acyl-coenzyme A to an alcohol via an aldehyde intermediate; this is coupled to the oxidation of two NADH molecules to maintain the NAD+ pool during fermentative metabolism. The structure of the alcohol dehydrogenase (ADH) domain of an ADHE protein from the ethanol-producing thermophile Geobacillus thermoglucosidasius has been determined to 2.5 Å resolution. This is the first structure to be reported for such a domain. In silico modelling has been carried out to generate a homology model of the aldehyde dehydrogenase domain, and this was subsequently docked with the ADH-domain structure to model the structure of the complete ADHE protein. This model suggests, for the first time, a structural mechanism for the formation of the large multimeric assemblies or `spirosomes' that are observed for this ADHE protein and which have previously been reported for ADHEs from other organisms.

Journal Keywords: Amino; Biofuels; Crystallography; X-Ray; Ethanol; Fermentation; Geobacillus; Models; Molecular; Multienzyme Complexes

Diamond Keywords: Biofuel; Bacteria; Enzymes

Subject Areas: Biology and Bio-materials, Energy, Chemistry

Instruments: I03-Macromolecular Crystallography

Added On: 24/09/2013 09:05

Discipline Tags:

Bioenergy Energy Biochemistry Catalysis Chemistry Structural biology Life Sciences & Biotech

Technical Tags:

Diffraction Macromolecular Crystallography (MX)