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Crystal structure of Venezuelan hemorrhagic fever virus fusion glycoprotein reveals a class 1 post-fusion architecture with extensive glycosylation

DOI: 10.1128/JVI.02298-13 DOI Help
PMID: 24049182 PMID Help

Authors: Marie-Laure Parsy (University of Oxford) , Karl Harlos (Wellcome Trust Centre for Human Genetics, University of Oxford) , Juha Huiskonen (University of Oxford) , Thomas Bowden (Division of Structural Biology, University of Oxford)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Journal of Virology

State: Published (Approved)
Published: September 2013

Open Access Open Access

Abstract: Guanarito virus (GTOV) is an emergent and deadly pathogen. We present the crystal structure of the glycosylated GTOV fusion glycoprotein to 4.1 Å-resolution, in the post-fusion conformation. Our structure reveals a classical six-helix bundle and presents direct verification that New World arenaviruses exhibit class-I viral membrane fusion machinery. The structure provides visualization of an N-linked glycocalyx coat and consideration of glycan dynamics reveals extensive coverage of the underlying protein surface, following virus−host membrane fusion.

Journal Keywords: Arenaviruses ; New; Cell; Crystallography ; X-Ray ; Glycosylation ; Hemorrhagic; American ; Humans ; Models ; Molecular ; Protein; Secondary ; Sequence; Viral; Virus Internalization

Subject Areas: Biology and Bio-materials, Medicine


Instruments: I04-Macromolecular Crystallography